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Protein-Protein Binding Kinetics by Biolayer Interferometry
The specific kinetics and thermodynamics of protein-protein interactions underlie the molecular mechanisms of cellular functions; hence the...
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Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system
EfeUOB/M has been characterised in Pseudomonas syringae pathovar. syringae as a novel type of ferrous-iron transporter, consisting of an...
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The critical role of a conserved lysine residue in periplasmic nitrate reductase catalyzed reactions
Periplasmic nitrate reductase NapA from Campylobacter jejuni ( C. jejuni ) contains a molybdenum cofactor (Moco) and a 4Fe–4S cluster and catalyzes the...
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Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria depends on delivery of unfolded OMPs to the...
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Protein–Protein Interactions: Oxidative Bacterial Two Hybrid
Protein–protein interaction studies are essential to understand how proteins organize themselves into interaction networks and thus influence...
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Directed evolution of tripartite ATP-independent periplasmic transporter for 3-Hydroxypropionate biosynthesis
AbstractOur previous study’s introduction of the malonic acid assimilation pathway into Escherichia coli enabled biosynthesis of 3-Hydroxypropionate...
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Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family
Most Gram-negative bacteria synthesize osmo-regulated periplasmic glucans (OPG) in the periplasm or extracellular space. Pathogenicity of many...
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Structural insights into the mechanism of protein transport by the Type 9 Secretion System translocon
Secretion systems are protein export machines that enable bacteria to exploit their environment through the release of protein effectors. The Type 9...
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Expression of a periplasmic β-carbonic anhydrase (CA) gene is positively correlated with HCO3- utilization by the gametophytes of Saccharina japonica (Phaeophyceae, Ochrophyta)
As an ecologically and economically important seaweed, Saccharina japonica has developed strategies to utilize HCO 3 - . In general, seaweeds have at...
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De Novo Designing a Novel Signal Peptide for Secretion of Neurturin to the Periplasmic Space of Escherichia coli
AbstractNeurotrophic factors, including neurturin, increase the life span of the neurons and prevent neuronal destruction. As recombinant expression...
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Thermodynamics of semi-specific ligand recognition: the binding of dipeptides to the E.coli dipeptide binding protein DppA
This investigation of the temperature dependence of DppA interactions with a subset of three dipeptides (AA. AF and FA) by isothermal titration...
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Bacterial divisome protein FtsA forms curved antiparallel double filaments when binding to FtsN
During bacterial cell division, filaments of tubulin-like FtsZ form the Z-ring, which is the cytoplasmic scaffold for divisome assembly. In Escherichia...
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Modeling of MT. P495, an mRNA-based vaccine against the phosphate-binding protein PstS1 of Mycobacterium tuberculosis
Tuberculosis (TB) is a contagious disease that predominantly affects the lungs, but can also spread to other organs via the bloodstream. TB affects...
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The Pseudomonas aeruginosa substrate-binding protein Ttg2D functions as a general glycerophospholipid transporter across the periplasm
In Pseudomonas aeruginosa , Ttg2D is the soluble periplasmic phospholipid-binding component of an ABC transport system thought to be involved in...
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When less is more: shortening the Lpp protein leads to increased vancomycin resistance in Escherichia coli
Vancomycin is a naturally occurring cell-wall-targeting glycopeptide antibiotic. Due to the low potency of this antibiotic against Gram-negative...
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Elucidating the zinc-binding proteome of Fusarium oxysporum f. sp. lycopersici with particular emphasis on zinc-binding effector proteins
Fusarium oxysporum f. sp. lycopersici is a soil-borne phytopathogenic species which causes vascular wilt disease in the Solanum lycopersicum ...
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Nutraceutical and Health-Promoting Potential of Lactoferrin, an Iron-Binding Protein in Human and Animal: Current Knowledge
Lactoferrin is a natural cationic iron-binding glycoprotein of the transferrin family found in bovine milk and other exocrine secretions, including...
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Protein Sorting Prediction
Many computational methods are available for predicting protein sorting in bacteria. When comparing them, it is important to know that they can be...
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Identification of Protein Partners by APEX2 Proximity Labeling
Proximity labeling methods enable the identification of proteins in the vicinity of a protein of interest in living cells. Among them, APEX2...
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Periplasmic synthesis and purification of the human prolactin antagonist Δ1-11-G129R-hPRL
The human prolactin antagonist Δ 1-11 -G129R-hPRL is a 21.9 kDa recombinant protein with 188 amino acids that downregulates the proliferation of a...
