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Measurement of Mitochondrial (Dys)Function in Cellular Systems Using Electron Paramagnetic Resonance (EPR): Oxygen Consumption Rate and Superoxide Production
The oxygen consumption rate (OCR) and superoxide production are crucial when assessing mitochondrial function and/or dysfunction. EPR spectroscopy...
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Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy
Outer membrane proteins (OMPs) of Gram-negative bacteria are involved in many essential functions of the cell. They are tightly packed in the outer...
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The use of EPR spectroscopy to study transcription mechanisms
Electron paramagnetic resonance (EPR) spectroscopy has become a promising structural biology tool to resolve complex and dynamic biological...
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PELDOR Measurements on Nitroxide-Labeled Oligonucleotides
In the past decades, pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) has emerged as a powerful tool in biophysical chemistry to...
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Methods to Measure Reactive Oxygen Species Production by NADPH Oxidases
NADPH oxidases (Noxes) are a family of enzymes that catalyze the oxidation of NADPH by molecular oxygen to produce superoxide radical anion (O2•–)...
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Wavelength, dose, skin type and skin model related radical formation in skin
The exposure to sun radiation is indispensable to our health; however, a long-term and high exposure could lead to cell damage, erythema, premature...
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Application of electron paramagnetic resonance spectroscopy for determining the relative nanoenvironment fluidity of polymeric micelles
AbstractPolymeric micelles are nanocarriers for drug, protein and gene delivery due to their unique core/shell structure, which encapsulates and...
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Studies of transmembrane peptides by pulse dipolar spectroscopy with semi-rigid TOPP spin labels
Electron paramagnetic resonance (EPR)-based pulsed dipolar spectroscopy measures the dipolar interaction between paramagnetic centers that are...
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Singlet Oxygen and Superoxide Anion Radical Detection by EPR Spin Trap** in Thylakoid Preparations
Reactive oxygen species (ROS) are produced by energy transfer and electron transport in plant chloroplast thylakoids at non-toxic levels under normal...
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EPR Spectroscopy as a Method for ROS Quantification in the Skin
Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for...
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Tuning flavin environment to detect and control light-induced conformational switching in Drosophila cryptochrome
Light-induction of an anionic semiquinone (SQ) flavin radical in Drosophila cryptochrome (dCRY) alters the dCRY conformation to promote binding and...
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An EPR Study Using Cyclic Hydroxylamines To Assess The Level of Mitochondrial ROS in Superinvasive Cancer Cells
It has been proposed that a mitochondrial switch involving a high mitochondrial superoxide production is associated with cancer metastasis. We here...
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Clinical Probes for ROS and Oxidative Stress
Electron paramagnetic resonance (EPR) spectroscopy is considered as a valuable tool in the determination and characterization of free radicals in...
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Spin Labeling of Long RNAs Via Click Reaction and Enzymatic Ligation
Electron paramagnetic resonance (EPR) is a spectroscopic method for investigating structures, conformational changes, and dynamics of...
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Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana
Nitroxyl (HNO) is the one-electron reduced and protonated congener of nitric oxide (•NO), owning a distinct chemical profile. Based on real-time...
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Interplay of oxidative stress, cellular communication and signaling pathways in cancer
Cancer remains a significant global public health concern, with increasing incidence and mortality rates worldwide. Oxidative stress, characterized...
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Heterocyclic Resveratrol Analogs. Synthesis and Physiological Activity: Part 1–Analogs Obtained by the Replacement of Aryl Residues with Heterocyclic Fragments
AbstractResveratrol is a polyphenolic phytoalexin, a stilbene derivative, whose physiological activities have been studied in a large number of...
