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  1. Cell Fractionation

    Protein function is generally dependent on its subcellular localization. In gram-negative bacteria such as Escherichia coli, a protein can be...
    Melissa Petiti, Laetitia Houot, Denis Duché in Bacterial Secretion Systems
    Protocol 2024
  2. Bacterial Envelope Fractionation

    Numerous bioinformatics tools allow predicting the localization of membrane proteins in the outer or inner membrane of Escherichia coli with high...
    Athanasios Saragliadis, Dirk Linke in Transmembrane β-Barrel Proteins
    Protocol 2024
  3. Fractionation of selenium isotopes during biofortification of Saccharomyces cerevisiae and the influence of metabolic labeling with 15N

    Isotope fractionation of metals/metalloids in biological systems is an emerging research area that demands the application of state-of-the-art...

    Luis Fernando Mejia Diaz, Jakub Karasinski, ... Katarzyna Wrobel in JBIC Journal of Biological Inorganic Chemistry
    Article 30 August 2023
  4. Fractionation Strategies

    Lignocellulosic biomass has recently attracted attention as a promising option for replacing non-renewable resources such as fossil fuels with...
    Diep Trung Tin Le, Tae Hyun Kim in Biomass Utilization: Conversion Strategies
    Chapter 2022
  5. Nitrogen Assimilation and Fractionation Dynamics in Wheat Genotypes Grown in Different Mediums

    The optimum growth and development of wheat depend on nitrogen (N) from different sources and its assimilation mechanisms. Experiments were conducted...

    Debajyoti Paul, Sravanthi Satuluri, Subodh Kumar Sinha in Journal of Plant Growth Regulation
    Article 20 May 2023
  6. Effects of Fractionation Methods on Soil Aggregate Microbial Community Composition: Settling vs. Wet Sieving

    Soil microbial communities play a vital role in biogeochemical cycling and ecosystem functions at the aggregate scale. Soil fractionation methods can...

    **n Gao, Rui Wang, ... ** Huang in Journal of Soil Science and Plant Nutrition
    Article 12 January 2024
  7. Foam fractionation Tags (F-Tags) enabling surfactant-free, activity-preserving recovery of enzymes

    Abstract

    Enzymes have become important tools in many industries. However, the full exploitation of their potential is currently limited by a lack of...

    Thomas Krause, Behnam Keshavarzi, ... Marion B. Ansorge-Schumacher in Applied Microbiology and Biotechnology
    Article Open access 17 January 2024
  8. Capillary Electrophoresis Plasma Fractionation for Lipids Analysis

    Extraction of lipids has mainly used density liquid phase separation techniques; however, these methods are limited by their broad extraction and...
    Anddre Osmar Valdivia, Carolyn Cray, Sanjoy K. Bhattacharya in Lipidomics
    Protocol 2023
  9. Automated On-Line Isolation and Fractionation Method for Subpopulations of Extracellular Vesicles

    Immunoaffinity chromatography (IAC) with selective antibodies immobilized on polymeric monolithic disk columns enables selective isolation of...
    Susanne K. Wiedmer, Evgen Multia, ... Marja-Liisa Riekkola in Cell-Secreted Vesicles
    Protocol 2023
  10. Co-fractionation–mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain

    Human mitochondrial (mt) protein assemblies are vital for neuronal and brain function, and their alteration contributes to many human disorders,...

    Mara Zilocchi, Matineh Rahmatbakhsh, ... Mohan Babu in Nature Protocols
    Article 20 November 2023
  11. RuBisCO Depletion and Subcellular Fractionation for Enhanced Florigen Detection in Arabidopsis

    In plants, complex signaling networks monitor and respond to environmental cues to determine the optimal time for the transition from the vegetative...
    Katarzyna Gawarecka, Ji Hoon Ahn in Thermomorphogenesis
    Protocol 2024
  12. Heterogeneous Catalytic Fractionation of Birch-Wood Biomass into a Microcrystalline Cellulose, Xylose and Enterosorbents

    Abstract

    The main components of the birch wood were fractionated into a microcrystalline cellulose, xylose and enterosorbents by an integration of...

    B. N. Kuznetsov, N. V. Garyntseva, ... A. V. Pestunov in Russian Journal of Bioorganic Chemistry
    Article 28 December 2022
  13. Postphotosynthetic Fractionation in Leaves, Phloem and Stem

    Stable carbon isotopeCarbon isotope ratios (δ13C) in organic matterOrganic matter convey important integrated and (if assessed in the tree ring...
    Arthur Gessler, Juan Pedro Ferrio in Stable Isotopes in Tree Rings
    Chapter Open access 2022
  14. Bioassay-Guided Fractionation of Methanolic Leaf Extract of Cinnamomum tamala, GC–MS Analysis and In Silico Studies

    Plants are being used in treating diseases since the beginning of human civilization. Cinnamomum tamala is a traditionally used medicinal plant used...

    Article 13 June 2024
  15. Quantitative profiling of posttranslational modifications of pathological tau via sarkosyl fractionation and mass spectrometry

    Tau protein aggregation is associated with posttranslational modifications (PTMs) in 75% of all dementia cases. The distribution of tau pathology and...

    Kathrin Wenger, Arthur Viode, ... Judith A. Steen in Nature Protocols
    Article 30 January 2024
  16. Dissolved rare earth elements distribution and fractionation in a subtropical coastal river: a case study from Jiulong River, Southeast China

    Dissolved rare earth elements (REE) can reflect the environmental changes in river systems and trace the mixing processes between rivers and the...

    Shunrong Ma, Guilin Han, ... **aoqiang Li in Aquatic Ecology
    Article 20 July 2023
  17. Discovering Protein–Protein Interactions using Co-Fractionation-Mass Spectrometry with Label-Free Quantitation

    Proteins generally achieve their functions through interactions with other proteins, so being able to determine which proteins interact with which...
    Mopelola O. Akinlaja, R. Greg Stacey, ... Leonard J. Foster in Protein-Protein Interactions
    Protocol 2023
  18. Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation

    This procedure provides a comprehensive method for isolating double-stranded RNA (dsRNA) that relies on the different solubility of various nucleic...
    Mengzhu Chai, **nyue Ma, ... **aofei Cheng in Double-Stranded RNA
    Protocol 2024
  19. Comparison of Zimmermann and Six Fractionation Methods Aimed at Distinguishing Between Active, Slow, and Passive Pools of Soil Organic Matter

    Recently, soil organic matter (SOM) fractionation has become an important tool to separate SOM pools with different stability and decomposability....

    Adnan Mustafa, Martin Bartuška, ... Jan Frouz in Journal of Soil Science and Plant Nutrition
    Article 24 May 2022
  20. Silicon isotope fractionation dynamics during uptake and translocation by various crop species under three soil types

    Purpose

    Silicon is important for both plant growth and the soil biogeochemical cycle. Si uptake and accumulation ability varies in plants, which are...

    **gjie Zhou, Sheng Tang, ... Lianghuan Wu in Plant and Soil
    Article 03 February 2022
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