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Methods of Cryptococcal Polysaccharide Analysis Using ELISA
One of the standard assays for the fungal pathogen Cryptococcus neoformans is the glucuronoxylomannan (GXM) ELISA. This assay utilizes monoclonal...
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Extraction and Quantification of Nodularins from Shrimp Samples by ELISA
Nodularins are potent hepatotoxic cyanotoxins produced by harmful algal blooms (HABs). Aquaculture is boosting the demand for faster and more...
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Application of Nano-ELISA in Food Analysis
ELISA is a widely applied technique with good reliability, sensitivity, and specificity. Compared to other immunoassays, ELISA has been intensively...
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Detection of Tomato Spotted Wilt Virus (TSWV) Infection in Plants Using DAS-ELISA and Dot-ELISA
Plant viruses cause severe damages to crop productions each year worldwide. To prevent the losses caused by plant viruses, it is necessary to develop...
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DNA–Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding
Dysregulation of peroxisome proliferator-activated receptor (PPAR)-γ has been described in a plethora of pathological conditions, such as diabetes,...
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An Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Circulating Pi*Z Alpha1-Antitrypsin Polymers
Enzyme-linked immunosorbent assay (ELISA) is a sensitive immunoassay based on specific antigen–antibody reaction that is used for...
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Resistin as a potential diagnostic biomarker for sepsis: insights from DIA and ELISA analyses
PurposeThe primary objective of this investigation is to systematically screen and identify differentially expressed proteins (DEPs) within the...
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Proteoglycans: A Tool for Detecting Hyaluronan by ELISA-Like Methods
Hyaluronan is a non-sulfated glycosaminoglycan synthesized on the plasma membrane of almost all mammalian cells, which can interact with different...
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Development of recombinant capsid protein-based single serum dilution ELISA for sero-detection of porcine circovirus type 2 infection in pigs
Porcine Circovirus 2 (PCV2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS) of swine and is one of the reasons for...
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Human and camel cystic echinococcosis – a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification
Cystic echinococcosis (CE) is an emergent neglected disease affecting human and animals in Egypt with a wide distribution and incidence. This study...
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Establishment of an indirect ELISA for Mycobacterium tuberculosis MTB39A protein antibody
AbstractThe MTB39A protein is a member of the unique Mycobacterium tuberculosis ( MTB ) PE/PPE protein family and is the main candidate for...
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A candidate competitive ELISA based on monoclonal antibody 3A8 for diagnosis of contagious bovine pleuropneumonia
AbstractFor the development of a competitive ELISA (cELISA) to detect serum antibodies against the Mycoplasma mycoides subsp. Mycoides ( Mmm ) (strain...
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Sensitivities of a Rapid Test Versus an ELISA Kit for Detecting Anti-SARS-CoV-2 IgM/IgG in Sera from an Egyptian Cohort
This study aimed to compare diagnostic sensitivities of a rapid test (Rt) and an ELISA kit for detecting anti-SARS-CoV-2 IgM/IgG in...
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Epitope map** and establishment of a blocking ELISA for mAb targeting the p72 protein of African swine fever virus
AbstractThe African swine fever virus (ASFV) has the ability to infect pigs and cause a highly contagious acute fever that can result in a mortality...
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Comparison of ELISA and RIA methods to quantify arginine vasopressin hormone levels in cell culture
The radioimmunoassay (RIA) method is widely used to determine the levels of arginine vasopressin (AVP) in studies, especially in cell culture...
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Evaluation of ELISA and immunoaffinity fluorometric analytical tools of four mycotoxins in various food categories
Mycotoxins (MTs) are secondary toxic metabolites that can contaminate food, impacting quality and safety, leading to various negative health effects...
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Constructing an ELISA for Detection of Anti-Borrelia in Wildlife and Agricultural Animals
Zoonotic diseases have major impacts on human and animal health, as well as being ecologically significant. Lyme Borreliosis or Lyme disease, caused...
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A neutralizing monoclonal antibody–based blocking ELISA to detect bovine herpesvirus 1 and vaccination efficacy
AbstractInfections caused by bovine herpesvirus 1 (BoHV-1) remain a serious global issue to the health and welfare of the bovine industry. Monitoring...
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Designing a Novel Spike Linear B-Cell Epitopes (SLBE) Protein with High Performance in Sars-Cov2-ELISA Test
SARS-CoV2 spike glycoprotein acting a key role in the infection and transmission of coronaviruses. Spike glycoprotein is the target for the...
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Highly sensitive therapeutic drug monitoring of infliximab in serum by targeted mass spectrometry in comparison to ELISA data
BackgroundPresently, antibody concentration measurements for patients undergoing treatment are predominantly determined by ELISA, which still comes...
