Abstract
Hyaluronan is a non-sulfated glycosaminoglycan synthesized on the plasma membrane of almost all mammalian cells, which can interact with different proteoglycans of the extracellular matrix. Aggrecan, versican, neurocan, and brevican are proteoglycans whose structures present a specific protein domain called “link module,” which allows hyaluronan binding. Therefore, they can be helpful for assays that detect hyaluronan. For example, ELISA-like methods developed to measure hyaluronan amounts in solution are based on specific interactions between this molecule and the link module present in aggrecan or other hyaluronan-binding proteins (hyaladherins).
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Acknowledgments
This work was in part supported by PICTO UNNOBA 2019 N 00011.
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Vitale, D.L., Cordo-Russo, R.I., Alaniz, L. (2023). Proteoglycans: A Tool for Detecting Hyaluronan by ELISA-Like Methods. In: Karamanos, N.K. (eds) Proteoglycans. Methods in Molecular Biology, vol 2619. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2946-8_3
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DOI: https://doi.org/10.1007/978-1-0716-2946-8_3
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