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Collateral activity of the CRISPR/RfxCas13d system in human cells
CRISPR/Cas13 systems are increasingly used for programmable targeting of RNAs. While Cas13 nucleases are capable of degrading both target RNAs and...
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The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model
BackgroundThe CRISPR-Cas13 system is an RNA-guided RNA-targeting system and has been widely used in transcriptome engineering with potentially...
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DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity
Two prokaryotic defence systems, prokaryotic Argonautes (pAgos) and CRISPR–Cas, detect and cleave invader nucleic acids using complementary guides...
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High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects
CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various organisms. However, collateral degradation of bystander RNAs has...
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Antibiotic-induced collateral damage to the microbiota and associated infections
Antibiotics have transformed medicine, saving millions of lives since they were first used to treat a bacterial infection. However, antibiotics...
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Metabolic collateral lethal target identification reveals MTHFD2 paralogue dependency in ovarian cancer
Recurrent loss-of-function deletions cause frequent inactivation of tumour suppressor genes but often also involve the collateral deletion of...
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Forest Stands as Collateral in Indonesia
The use of low or non-risk assets as collateral is common practice. However, if only the forest stand, which does not include the land where the...
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Collateral lethality between HDAC1 and HDAC2 exploits cancer-specific NuRD complex vulnerabilities
Transcriptional co-regulators have been widely pursued as targets for disrupting oncogenic gene regulatory programs. However, many proteins in this...
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Local transplantation of mesenchymal stem cells improves encephalo-myo-synangiosis-mediated collateral neovascularization in chronic brain ischemia
BackgroundTo explore whether local transplantation of mesenchymal stem cells (MSCs) in temporal muscle can promote collateral angiogenesis and to...
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Collateral Effects of Nanopollution on Human and Environmental Health
The development of nanotechnology in the field of agriculture and environment during recent times has significantly advanced the area and paved the...
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Collateral beauty in the damages: an overview of cosmetics and therapeutic applications of microbial proteases
Microbial proteases are enzymes secreted by a variety of microorganisms, including bacteria and fungi, and have attracted significant attention due...
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Identifying collateral and synthetic lethal vulnerabilities within the DNA-damage response
BackgroundA pair of genes is defined as synthetically lethal if defects on both cause the death of the cell but a defect in only one of the two is...
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Single Stage Purification of CRISPR/Cas13a Nuclease via Metal-Chelating Chromatography Following Heterologous Expression with the Preservation of Collateral Ribonuclease Activity
AbstractCRISPR/Cas13a nucleases are currently considered to be the basis for the development of a new generation of biosensors for the...
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Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing
The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short...
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Monoterpene indole alkaloids with anticancer activity from Tabernaemontana species
Indole alkaloids, predominantly found in plants, are a large group of natural product-derived compounds characterized by a remarkable chemical...
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Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning
Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors depend on accurate prediction of on-target activity and...
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Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector
The recently discovered type III-E CRISPR-Cas effector Cas7-11 shows promise when used as an RNA manipulation tool, but its structure and the...
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Reward expectation enhances action-related activity of nigral dopaminergic and two striatal output pathways
Neurons comprising nigrostriatal system play important roles in action selection. However, it remains unclear how this system integrates recent...
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Trans-nuclease activity of Cas9 activated by DNA or RNA target binding
Type V and type VI CRISPR–Cas systems have been shown to cleave nonspecific single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA) in trans , but...
