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Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry
An optimized Bligh and Dyer protocol and subsequent derivatization is described in this chapter for the extraction of free cholesterol and...
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Raman microscopy-based quantification of the physical properties of intracellular lipids
The physical properties of lipids, such as viscosity, are homeostatically maintained in cells and are intimately involved in physiological roles....
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Algal lipids for biofuel production: strategies, environmental impacts, downstream processing and commercialization
The demand for fossil fuels has resulted in their rapid depletion and rise in the fuel costs. Moreover, fossil fuels have serious negative impacts on...
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Multi-omics profiling of PC-3 cells reveals bufadienolides-induced lipid metabolic remodeling by regulating long-chain lipids synthesis and hydrolysis
IntroductionLipid metabolism participates in various biological processes such as proliferation, apoptosis, migration, invasion, and maintenance of...
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Membrane Lipids and Osmolytes Composition of Xerohalophilic Fungus Aspergillus penicillioides during Growth on High NaCl and Glycerol Media
AbstractAspergillus penicillioides may be both xerophilic and halophilic. To study whether there is a difference in the mechanisms of adaptation to...
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Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for the Analysis of (Phospho)Lipids
Lipids are important and abundant constituents of all biological tissues and body fluids. In particular, phospholipids (PLs) constitute a major part...
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Roles of Antioxidant Enzymes, Secondary Metabolites, and Lipids in Light Adaption of Tea-Oil Plant (Camellia oleifera Abel)
Tea-oil ( Camellia oleifera Abel) is an important woody crop for producing high-quality edible oil, which has been extensively cultivated in an...
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An essential vesicular-trafficking phospholipase mediates neutral lipid synthesis and contributes to hemozoin formation in Plasmodium falciparum
BackgroundPlasmodium falciparum is the pathogen responsible for the most devastating form of human malaria. As it replicates asexually in the...
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Neutral sphingomyelinase inhibition promotes local and network degeneration in vitro and in vivo
BackgroundCell-to-cell communication is vital for tissues to respond, adapt, and thrive in the prevailing milieu. Several mechanisms mediate...
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Lipids of Indo-Pacific gorgonian corals are modified under the influence of microbial associations
Gorgonian corals form complex interactions with a wide range of microorganisms, which play a key role in maintaining health of the holobiont. To...
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Oral Processing of Lipids
This chapter deals with fundamental knowledge obtained so far, about our understanding of oral processing of dietary lipids. Comprehensive knowledge...
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Alterations in complex lipids in tumor tissue of patients with colorectal cancer
BackgroundAccumulating evidence indicates alterations in lipid metabolism and lipid composition in neoplastic tissue. Earlier nuclear magnetic...
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Production of a food grade extract of Chlorococcum amblystomatis rich in omega-3 lipids using ethanol assisted with ultrasound and deep characterization by lipidomics
Chlorococcum amblystomatis is a novel microalga with a high content of omega-3 fatty acids (FAs), with applications for food, feed, nutraceuticals,...
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Critical assessment of the sulfo-phospho-vanillin method to quantify lipids in freeze-dried microalgae
The sulfo-phospho-vanillin assay is a well-known method to measure the lipid content of microalgae. Following hydrolysis with sulfuric acid, a pink...
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ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis
Plant ER membranes are the major site of biosynthesis of several lipid families (phospholipids, sphingolipids, neutral lipids such as sterols and...
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Insights into membrane lipids modification in barley leaves as an adaptation mechanism to cold stress
The membranes of plants are where temperature sensing begins and where freezing injury typically occurs. Barley’s adaptation to and survival after...
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Lipid droplets and cellular lipid flux
Lipid droplets are dynamic organelles that store neutral lipids, serve the metabolic needs of cells, and sequester lipids to prevent lipotoxicity and...
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Lipids in Porcupine Quills as Putative Sources of Communicatory Signals
Specialized scent glands associated with modified epidermal hairs such as quills and spines serve as a putative source of pheromone in many mammals....
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Comparison of the functions of plasma membrane and vacuolar membrane lipids in plant cell protection against hyperosmotic stress
Main conclusionThe comparison of the changes of the lipid content in plant cell boundary membranes demonstrates a substantial role of the vacuolar...
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Collection and Analysis of Phloem Lipids
The plant phloem is a long-distance conduit for the transport of assimilates but also of mobile developmental and stress signals. These signals can...