SpringerLink
Log in

ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis

  • Protocol
  • First Online:
The Plant Endoplasmic Reticulum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2772))

  • 362 Accesses

Abstract

Plant ER membranes are the major site of biosynthesis of several lipid families (phospholipids, sphingolipids, neutral lipids such as sterols and triacylglycerols). The structural diversity of lipids presents considerable challenges to comprehensive lipid analysis. This chapter will briefly review the various biosynthetic pathways and will detail several aspects of the lipid analysis: lipid extraction, handling, separation, detection, identification, and data presentation. The different tools/approaches used for lipid analysis will also be discussed in relation to the studies to be carried out on lipid metabolism and function.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Morré DJ, Panel C, Morré DM, Sandelius AS, Moreau P, Andersson B (1991) Cell-free transfer and sorting of membrane lipids in spinach. Donor and acceptor specificity. Protoplasma 160:49–64

    Article  Google Scholar 

  2. Parsons HT, Drakakaki G, Heazlewood JL (2013) Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network. Front Plant Sci 3:298

    Article  PubMed  PubMed Central  Google Scholar 

  3. Parsons HT, Fernández-Niño SM, Heazlewood JL (2014) Separation of the plant Golgi apparatus and endoplasmic reticulum by free-flow electrophoresis. Methods Mol Biol 1072:527–539

    Article  CAS  PubMed  Google Scholar 

  4. Li-Beisson Y, Shorrosh B, Beisson F, Andersson MX, Arondel V, Bates PD, Baud S, Bird D, DeBono A, Durrett TP, Franke RB, Graham IA, Katayama K, Kelly AA, Larson T, Markham JE, Miquel M, Molina I, Nishida I, Rowland O, Samuels L, Schmid KM, Wada H, Welti R, Changcheng X, Zallot R, Ohlrogge J (2013) Acyl-lipid metabolism. In: The Arabidopsis book, vol 11, p e0161

    Google Scholar 

  5. Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497–509

    Article  CAS  PubMed  Google Scholar 

  6. Heape AM, Juguelin H, Boiron F, Cassagne C (1985) Improved one dimensional thin layer chromatographic technique for polar lipids. J Chromatogr 332:391–395

    Article  Google Scholar 

  7. Macala LJ, Yo RK, Ando S (1983) Analysis of brain lipids by high performance TLC and densitometry. J Lipid Res 24:1243–1250

    Article  CAS  PubMed  Google Scholar 

  8. Laloi M, Perret AM, Chatre L, Melser S, Cantrel C, Vaultier MN, Zachowski A, Bathany K, Schmitter JM, Vallet M, Lessire R, Hartmann MA, Moreau P (2007) Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells. Plant Physiol 143:461–472

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Cacas JL, Melser S, Domergue F, Joubès J, Bourdenx B, Schmitter JM, Mongrand S (2012) Rapid nanoscale quantitative analysis of plant sphingolipid long-chain bases by GC-MS. Anal Bioanal Chem 403:2745–2755

    Article  CAS  PubMed  Google Scholar 

  10. Hillig I, Leipelt M, Ott C, Zahringer U, Warnecke D, Heinz E (2003) Formation of glucosylceramides and sterol glucoside by a UDP-glucose-dependent glucosylceramide synthase from cotton expressed in Pichia pastoris. FEBS Lett 553:365–369

    Article  CAS  PubMed  Google Scholar 

  11. Ito Y, Esnay N, Platre MP, Wattelet-Boyer V, Noack LC, Fougère L, Menzel W, Claverol S, Fouillen L, Moreau P, Jaillais Y, Boutté Y (2021) Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network. Nat Commun 12(1):4267. https://doi.org/10.1038/s41467-021-24548-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Juguelin H, Heape MA, Boiron F, Cassagne C (1986) A quantitative developmental study of neutral lipids during myelinogenesis in the peripheral nervous system of normal and trembler mice. Brain Res 390:249–252

    Article  CAS  PubMed  Google Scholar 

  13. Brown DJ, Dupont FM (1989) Lipid composition of plasma membranes and endomembranes prepared from roots of barley (Hordeum vulgare L.): effects of salt. Plant Physiol 90:955–961

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Grindstaff KK, Fielding LA, Brodl MR (1996) Effect of gibberellin and heat shock on the lipid composition of endoplasmic reticulum in barley aleurone layers. Plant Physiol 110:571–581

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Moreau P, Bessoule JJ, Mongrand S, Testet E, Vincent P, Cassagne C (1998) Lipid trafficking in plant cells. Progr Lipid Res 37:371–339

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Bordeaux Metabolome Facility, MetaboHUB (ANR-11-INBS-0010).

Author information

Authors and Affiliations

  1. CNRS-University of Bordeaux, UMR 5200 Membrane Biogenesis Laboratory, INRAe Bordeaux Aquitaine, Villenave d’Ornon, France

    Laetitia Fouillen, Lilly Maneta-Peyret & Patrick Moreau

  2. Bordeaux Imaging Center, UMS 3420 CNRS, US004 INSERM, University of Bordeaux, Bordeaux, France

    Patrick Moreau

Authors
  1. Laetitia Fouillen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lilly Maneta-Peyret
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patrick Moreau
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Patrick Moreau .

Editor information

Editors and Affiliations

  1. Biological and Medical Sciences, Oxford Brookes University, Oxford, UK

    Verena Kriechbaumer

Rights and permissions

Reprints and permissions

Copyright information

© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Fouillen, L., Maneta-Peyret, L., Moreau, P. (2024). ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis. In: Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum. Methods in Molecular Biology, vol 2772. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3710-4_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-3710-4_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3709-8

  • Online ISBN: 978-1-0716-3710-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation