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Generation of Genome-Edited Mice by Cytoplasmic Injection of CRISPR-Cas9 RNA
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) (CRISPR-Cas9) gene editing enables rapid...
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Plastid genome data provide new insights into the dynamic evolution of the tribe Ampelopsideae (Vitaceae)
BackgroundAmpelopsideae J. Wen & Z.L. Nie is a small-sized tribe of Vitaceae Juss., including ca. 47 species from four genera showing a disjunct...
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Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line
Clustered regularly interspaced short palindromic repeats (CRISPR) technology is a powerful genome editing tool. Recently developed CRISPR-Cas12a...
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Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector
The clustered regularly interspersed short palindromic repeats (CRISPR)/Cas systems have become the most widely adopted genome editing platform owing...
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Elucidating the multichromosomal structure within the Brasenia schreberi mitochondrial genome through assembly and analysis
Brasenia schreberi , a plant species traditionally utilized in Chinese medicine and cuisine, represents an early evolutionary stage among flowering...
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Genoty** of Campylobacter jejuni Isolates from Poultry by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)
Campylobacter jejuni is the leading bacterial foodborne pathogen that causes human acute gastrointestinal illness worldwide. Due to its genetic...
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CRISPR-Cas: A History of Discovery and Innovation
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated [Cas]) is a rapidly evolving RNA-mediated adaptive immune...
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Investigation of the Genetic Diversity of Erwinia amylovora Strains Originating from Pome Fruits in Turkey
The present study addresses the molecular characterization of a collection of Erwinia amylovora strains obtained in 2019 from different locations in...
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Genome Editing Mediated by Primordial Germ Cell in Chicken
Genome editing technology has facilitated the studies on exploring specific gene functions in diverse living organisms. The technology has also...
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Comparative analyses and phylogenetic relationships of thirteen Pholidota species (Orchidaceae) inferred from complete chloroplast genomes
BackgroundThe orchid genus Pholidota Lindl. ex Hook. is economically important as some species has long been used in traditional medicine. However,...
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Characterization of the complete chloroplast genome of purple flowering stalk (Brassica campestris var. purpuraria) and comparisons with related species
Purple flowering stalk ( Brassica campestris var. purpuraria ) is an important vegetable and is widely distributed in the region of the Yangtze River....
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Genome-Editing Technologies in Crop Improvement
Global climate change, together with harmful biotic and abiotic factors, is limiting agricultural yields, rendering it challenging to meet the rising...
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Genome Editing by Ribonucleoprotein Based Delivery of the Cas9 System in Plants
Clustered regularly interspaced palindromic repeat (CRISPR)-based gene editing technology has opened the doors for targeted mutagenesis in a variety...
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A CRISPR/Cas9 Protocol for Target Gene Editing in Barley
Previous studies of gene function rely on the existing natural genetic variation or on induction of mutations by physical or chemical mutagenesis....
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Updated Overview of TALEN Construction Systems
Transcription activator-like effector (TALE) nuclease (TALEN) is the second-generation genome editing tool consisting of TALE protein containing...
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Chloroplast genome analyses of Caragana arborescens and Caragana opulens
BackgroundNumerous species within the genus Caragana have high ecological and medicinal value. However, species identification based on...
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Genome Editing and Biological Weapons Assessing the Risk of Misuse
This monograph introduces current genome editing technologies—clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated...
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Role of CRISPR-Cas and Its Application in Mitigating Plant Stress
Studies on stress physiology in plants are gradually becoming a priority for every researcher due to its direct effect on crop productivity,...
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Insights into the multi-chromosomal mitochondrial genome structure of the xero-halophytic plant Haloxylon Ammodendron (C.A.Mey.) Bunge ex Fenzl
BackgroundHaloxylon ammodendron holds significance as an ecological plant, showcasing remarkable adaptability to desert conditions, halophytic...
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Complete plastome genomes of three medicinal heliotropiaceae species: comparative analyses and phylogenetic relationships
BackgroundHeliotropiaceae is a family of the order Boraginales and has over 450 species. The members of the family Heliotropiaceae have been widely...
