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Staining Methods for Normal and Regenerative Myelin in the Nervous System
Histochemical and fluorescence-based techniques enable the specific identification of myelin by bright-field or fluorescence microscopy. In this...
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Isolation and Cultivation and Staining of Paramecium
Paramecium is the microscopic, unicellular eukaryotic biological indicator organism explored for the study of freshwater ecotoxicity and various...
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Staining to machine learning: An emerging technology for determination of microalgal cell viability
Microalgae are unicellular photosynthetic microorganisms typically found in aquatic environments and they play a vital role in the global carbon and...
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Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity
Bacterial capsular polysaccharides are important vaccine immunogens. However, the study of polysaccharide-specific immune responses has been hindered...
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Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes
Silver nitrate staining to evidence the location of nucleolar organizer regions (Ag-NORs) in chromosomes is widely used as a classical method in...
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Uranyl-Free Staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy
Uranyl acetate solution has widely been used as staining reagent for samples processed for ultrastructural morphology, cytochemistry, and...
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Detection of dsRNA by Acridine Orange Staining
Acridine orange is a nucleic acid binding dye that emits green fluorescence when bound to double-stranded DNA or RNA and red fluorescence when bound...
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Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians
The capability to simultaneously apply different molecular tools to visualize a wide variety of changes in genetic expression and tissue composition...
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Screening for Anticancer Activity: Dual Staining Method
Tumor is one of the most significant concerns worldwide. Recent studies have disclosed that through different pathways, bacterial extracts exhibit...
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TEM: Sample Preparation, Fixation, and Staining of Protists
Transmission electron microscopy (TEM) is an ideal device to study the internal structure of cells and different types of biological materials, as...
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Co-staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-Seq
The paired detection of the transcriptome and proteome at single-cell resolution provides exquisite insight to immune mechanisms in health and...
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Antibody Staining for Nematodes with Heat-induced Antigen Retrieval (HIAR)
Immunocytochemistry remains a valuable and necessary tool for biologists working with nematodes, even those nematode model organisms with advanced...
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High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain...
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Evaluation of a low-cost staining method for improved visualization of sweet potato whitefly (Bemisia tabaci) eggs on multiple crop plant species
BackgroundThe sweet potato whitefly ( Bemisia tabaci ) is a globally important insect pest that damages crops through direct feeding and by...
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FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry
This chapter provides a protocol for a detailed evaluation of phytoplankton and nuisance cyanobacteria with the FlowCam 8400 and the FlowCam Cyano....
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Comparative study of HA and HNB staining RT-LAMP assays for peste des petits ruminants virus detection in West African Dwarf goats
Peste des petits ruminants (PPR) cause severe economic losses to many countries of the world where the disease is endemic. It has been targeted for...
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Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy
Morphological characteristics of liposomes, such as size and lamellarity directly impact their quality and biological performance of encapsulated...
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High-Speed Clearing and High-Resolution Staining for Analysis of Various Markers for Neurons and Vessels
Background:Tissue clearing enables deep imaging in various tissues by increasing the transparency of tissues, but there were limitations of...
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Three-dimensional map** in multi-samples with large-scale imaging and multiplexed post staining
Dissection of the anatomical information at the single-cell level is crucial for understanding the organization rule and pathological mechanism of...
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Evaluation of karyotype diversity in Indian traditional aromatic rice cultivars through EMA-based non-fluorescent Giemsa and fluorescent DAPI staining
Rice serves as a primary dietary source of nutrients and calories for over half of the global human population. With India being the world's...
