Abstract
Silver nitrate staining to evidence the location of nucleolar organizer regions (Ag-NORs) in chromosomes is widely used as a classical method in plant cytogenetics. Here, we present the most used procedures and highlight some aspects in terms of their replicability by plant cytogeneticists. Some technical features described are materials and methods used, procedures, protocol modifications, and precautions in order to obtain positive signals. The methods to obtain Ag-NOR signals have different degrees of replicability, but do not require any sophisticated technology or equipment for their application.
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Acknowledgments
Our thanks to the Núcleo de Estudios Ambientales (NEA), and to the Dirección de Investigación, Universidad Católica de Temuco, Chile.
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Palma-Rojas, C., Jara-Seguel, P., Araya-Jaime, C. (2023). Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes. In: Heitkam, T., Garcia, S. (eds) Plant Cytogenetics and Cytogenomics. Methods in Molecular Biology, vol 2672. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3226-0_13
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DOI: https://doi.org/10.1007/978-1-0716-3226-0_13
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