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Tris(3-fluorophenyl)phosphine in reactions with acrylic acid and its derivatives: crystal structure and biological activity of the reaction products
New quaternary phosphonium salts were synthesized by the reactions of tris(3-fluorophenyl)phosphine with acrylic acid and its derivatives. The...
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A Stable and Sensitive TLC-Spray for Thiols, Disulfides and Thioesters
A sensitive and stable spray for thin layer chromatography of acyl-CoA and other thio-compounds has been evaluated. CoA, down to 1 nmol, is readily...
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A Selenium-based Cysteine Surrogate for Protein Chemical Synthesis
N-selenoethyl cysteine (SetCys) in the form of its cyclic selenosulfide is a cysteine surrogate, whose reactivity depends on the reducing power of...
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Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Tryptic Digest
The identification of proteins in samples of moderate to complex composition is primarily done by bottom-up approaches. Therefore, proteins are...
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Measuring the Reversible Oxidation of Protein Tyrosine Phosphatases Using a Modified Cysteinyl-Labeling Assay
The modified cysteinyl-labeling assay enables the labeling, enrichment, and detection of all members of the protein tyrosine phosphatase (PTP)...
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Synthesis of functionalized phosphorus-containing tetraorganosilanes
A convenient synthesis of functionalized β-phosphorus-substituted tetraorganosilanes containing hydrolysis-resistant organosilicon moieties comprises...
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Structural Flexibility of Tau in Its Interaction with Microtubules as Viewed by Site-Directed Spin Labeling EPR Spectroscopy
Tau is a microtubule-associated protein that belongs to the Intrinsically Disordered Proteins (IDPs) family. IDPs or Intrinsically Disordered Regions...
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Transient Co-expression of Membrane Protein Complexes in Mammalian Cells
Membrane proteins are essential components of biological membranes with key roles in cellular processes such as nutrient transport, cell...
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Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation
Glycoproteins obtained from cell culture supernatants or lysates generally exist as mixtures of over 100 differently glycosylated protein forms...
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Multidisciplinary Methods for Screening Toxic Proteins from Phages and Their Potential Molecular Targets
This chapter presents a comprehensive methodology for the identification, characterization, and functional analyses of potentially toxic hypothetical...
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Facile fabrication of durable antibacterial and anti-felting wool fabrics with enhanced comfort via novel N-phenylmaleimide finishing
In this study, we successfully synthesized N-phenylmaleimide (NPMI) and applied it to wool fabrics to obtain robust antimicrobial properties. First,...
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Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling
Low-input proteomics, which treats tens to hundreds of mammalian cells, is the gap between standard proteomics and single-cell proteomics. Low-input...
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Covalent Reactive Peptides to Block Protein–Protein Interactions and Inhibit Microbe–Host Interaction
A key step in enteropathogenic Escherichia coli (EPEC) infection of intestinal cells involves a Tir-induced actin reorganization. Nck mediates this...
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Preparation of Site-Specific Succinylated Histone Mimics to Investigate Its Impact on Nucleosome Dynamics
Posttranslational modifications (PTMs) of histones have been demonstrated to be the key regulating mechanism of nucleosome dynamics and chromatin...
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Preparation of Nucleic Acid Aptamer Functionalized Silver/Gold Nanoparticle Conjugates Using Thiol-Substituted Oligonucleotides
Silver and gold nanoparticle-aptamer conjugates have been extensively utilized as biosensors and microscopic vehicles that deliver a therapeutic...
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DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
In this chapter, we describe the utility of fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) as a proteomics platform for the...
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Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes
Cellular Communication Network (CCN) proteins are secretory growth factors often associated with extracellular matrix (ECM) and extracellular...
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Proteome Analysis of Whole Blood Collected by Volumetric Absorptive Microsampling
Proteomic biomarker discovery and analysis from human biofluids using liquid chromatography-mass spectrometry (LC-MS) is an area of intense...
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Preparation of Oxidized and Reduced PTP4A1 for Structural and Functional Studies
The formation of a reversible disulfide bond between the catalytic cysteine and a spatially neighboring cysteine (backdoor) in protein tyrosine...
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Analysis of the Saliva Proteome Using 2D-DIGE
Saliva is now an established biofluid with a number of important applications in use across research and clinical settings. Saliva contains an...