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Capillary Electrophoresis-Mass Spectrometry

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Christian Neusüß

    1. Aalen University, Aalen, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Kevin Jooß

    1. Aalen University, Aalen, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2531)

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About this book

This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.


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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Evaluation of Prototype CE-MS Interfaces

    • Sabrina Ferré, Julien Boccard, Serge Rudaz, Víctor González-Ruiz
    Pages 1-13

  3. Capillary Electrophoresis–Mass Spectrometry (CE-MS) by Sheath–Flow Nanospray Interface and Its Use in Biopharmaceutical Applications

    • Mei Han, Richard Smith, Dan A. Rock
    Pages 15-47

  4. Protocol for Etching Bare-Fused Silica Capillaries for Sheathless Capillary Electrophoresis–Mass Spectrometry Coupling

    • Clarisse Gosset-Erard, Jérémie Giorgetti, Michael Biacchi, Frédéric Aubriet, Emmanuelle Leize-Wagner, Patrick Chaimbault et al.
    Pages 49-59

  5. Microfabricated Liquid Junction Capillary Electrophoresis–Mass Spectrometry Interface

    • Jana Krenkova, Frantisek Foret
    Pages 61-68

  6. Successive Multiple Ionic-Polymer Layer Coatings for Intact Protein Analysis by Capillary Zone Electrophoresis–Mass Spectrometry: Application to Hemoglobin Analysis

    • Liesa Salzer, Alexander Stolz, Laura Dhellemmes, Alisa Höchsmann, Laurent Leclercq, Hervé Cottet et al.
    Pages 69-76

  7. On-line Immobilized Enzyme Microreactor Capillary Zone Electrophoresis–Mass Spectrometry for Peptide Map**

    • Roger Pero-Gascon, Laura Pont, Estela Giménez, Victoria Sanz-Nebot, Fernando Benavente
    Pages 77-91

  8. Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Tryptic Digest

    • Marius Segl, Hanno Stutz
    Pages 93-106

  9. Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Characterization of Proteoforms in Complex Samples

    • Elijah N. McCool, Rachele A. Lubeckyj, Daoyang Chen, Liangliang Sun
    Pages 107-124

  10. Assessment of Macro- and Microheterogeneity of Monoclonal Antibodies Using Capillary Zone Electrophoresis Hyphenated with Mass Spectrometry

    • Christoph Gstöttner, Rob Haselberg, Manfred Wuhrer, Govert W. Somsen, Elena Domínguez-Vega
    Pages 125-142

  11. High-Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS

    • W. Wang, G. S. M. Lageveen-Kammeijer
    Pages 143-162

  12. Capillary Zone Electrophoresis–Electrospray Ionization Tandem Mass Spectrometry for Total Analysis of Chondroitin/Dermatan Sulfate Oligosaccharides

    • Alina D. Zamfir
    Pages 163-184

  13. In-Source Fragmentation for the Identification of Compounds by CE-ESI-TOF in Human Plasma. l-Proline as Case Study

    • Maricruz Mamani-Huanca, Ana Gradillas, Ángeles López-Gonzálvez, Coral Barbas
    Pages 185-202

  14. Assessing the Energy Status of Low Numbers of Mammalian Cells by Capillary Electrophoresis–Mass Spectrometry

    • Wei Zhang, Rawi Ramautar
    Pages 203-209

  15. Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis

    • Elena Sánchez-López, María Luisa Marina
    Pages 211-225

  16. Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction Coupled to Capillary Electrophoresis–Tandem Mass Spectrometry

    • Francisco J. Lara, A. M. García-Campaña
    Pages 227-241

  17. CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis

    • Darya Mozhayeva, Carsten Engelhard
    Pages 243-257

  18. Back Matter

    Pages 259-260
    Download chapter PDF
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Editors and Affiliations

  • Aalen University, Aalen, Germany

    Christian Neusüß, Kevin Jooß

Bibliographic Information

  • Book Title: Capillary Electrophoresis-Mass Spectrometry

  • Book Subtitle: Methods and Protocols

  • Editors: Christian Neusüß, Kevin Jooß

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2493-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2492-0Published: 09 August 2022

  • Softcover ISBN: 978-1-0716-2495-1Published: 10 August 2023

  • eBook ISBN: 978-1-0716-2493-7Published: 08 August 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 260

  • Number of Illustrations: 1 b/w illustrations, 4 illustrations in colour

  • Topics: Mass Spectrometry, Biochemistry, general

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