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Synergistic promotion of transient transgene expression in CHO cells by PDI/XBP-1s co-transfection and mild hypothermia
Transient gene expression system is an important tool for rapid production of recombinant proteins in Chinese hamster ovary (CHO) cells. However,...
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Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
Large culture volumes are often required when expression constructs are particularly low-yielding or when end uses require significant amounts of...
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Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection
Large-scale transient expression in Chinese Hamster Ovary (CHO) cells provides a rapid protein production method with a potential start-to-end...
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PEI-Mediated Transient Gene Expression in CHO Cells
We describe a method for polyethyleneimine (PEI)-mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for...
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Flow Cytometry Quantification of Transient Transfections in Mammalian Cells
Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow...
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Optimized protocols for protoplast isolation, transfection, and regeneration in the Solanum genus for the CRISPR/Cas-mediated transgene-free genome editing
The Solanaceae family includes the largest flowering crops such as tomatoes, potatoes, and eggplants. Consumer demand has led to massive development...
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Transient Co-expression of Membrane Protein Complexes in Mammalian Cells
Membrane proteins are essential components of biological membranes with key roles in cellular processes such as nutrient transport, cell...
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DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent
Mammalian transient expression systems enable flexible and rapid production of proteins. They’re ideal for expression of human or other mammalian...
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Novel opto-fluidic drug delivery system for efficient cellular transfection
Intracellular drug delivery is at the heart of many diagnosis procedures and a key step in gene therapy. Research has been conducted to bypass cell...
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Single-Cell Exogenous Gene Transfection Analysis Chip
Fast and accurate profiling of exogenous gene expressionGene expression in host cells is crucial for studying gene function in cellular and molecular...
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Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein
BackgroundMammalian cell lines are frequently used as protein expression hosts because of their ability to correctly fold and assemble complex...
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Vibropolyfection: coupling polymer-mediated gene delivery to mechanical stimulation to enhance transfection of adherent cells
BackgroundWith the success of recent non-viral gene delivery-based COVID-19 vaccines, nanovectors have gained some public acceptance and come to the...
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Endpoint and Kinetic Approaches for Assessing Transfection Efficacy in Mammalian Cell Culture
The efficacy of transfection reagents and nanoparticles is often assessed by measuring levels of expressed reporter protein. Fluorescence and...
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Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of...
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Production and Purification of Adeno-Associated Viral Vectors (AAVs) Using Orbitally Shaken HEK293 Cells
Here, we describe methods for the production of adeno-associated viral (AAV) vectors by transient transfection of HEK293 cells grown in serum-free...
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Improved Manufacturing Methods of Extracellular Vesicles Pseudotyped with the Vesicular Stomatitis Virus Glycoprotein
Extracellular vesicles (EV), which expose the vesicular stomatitis virus glycoprotein (VSVG) on their surface, are used for delivery of nucleic acids...
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The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production...
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A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
BackgroundCytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research...
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A feasibility study of different commercially available serum-free mediums to enhance lentivirus and adeno-associated virus production in HEK 293 suspension cells
Lentivirus and adeno-associated viruses are invaluable tools for biotechnology applications due to their genetic material delivery abilities both...
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Transient light-activated gene expression in Chinese hamster ovary cells
BackgroundChinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and...
