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Isothermal circular strand displacement–based assay for microRNA detection in liquid biopsy
Extracellular miRNAs are promising targets for develo** new assays for the early diagnosis and prognosis of diseases based on liquid biopsy. The...
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A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch
Herein, we developed a novel fluorescent assay using spherical identification probes and toehold-mediated strand displacement reaction-initiated...
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Validation and Application of a Dried Blood Spot Amoxicillin Assay
Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) investigations in situations where venous blood sampling is logistically...
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Simple, sensitive, and label-free miRNA analysis through strand displacement reaction integrating with G-quadruplex-based signal generation
MicroRNAs (miRNAs) play a significant role in the pathogenesis of various diseases throughout biological processes, and the accurate detection of...
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A lateral flow assay for miRNA-21 based on CRISPR/Cas13a and MnO2 nanosheets-mediated recognition and signal amplification
The point-of-care testing (POCT) of miRNA has significant application in medical diagnosis, yet presents challenges due to their characteristics of...
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SF-qPCR: Strand Displacement-Based Fast Quantitative Polymerase Chain Reaction
Nucleic acid testing (NAT) is important for the identification and quantification of specific nucleic acid targets, both DNA and RNA, in life...
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Ratiometric electrochemical detection of miRNA based on DNA nanomachines and strand displacement reaction
MicroRNAs (miRNAs) play an important role in regulating gene expression in cells. Abnormal expression of miRNAs has been associated with a variety of...
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Strand displacement–triggered FRET nanoprobe tracking TK1 mRNA in living cells for ratiometric fluorimetry of nucleic acid biomarker
A precisely designed dual-color biosensor has realized a visual assessment of thymidine kinase 1 (TK1) mRNA in both living cells and cell lysates....
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Integrating CRISPR/Cas12a with strand displacement amplification for the ultrasensitive aptasensing of cadmium(II)
Cadmium ion (Cd(II)) is a pernicious environmental pollutant that has been shown to contaminate agricultural lands, accumulate through the food...
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Programming cell-free biosensors with DNA strand displacement circuits
Cell-free biosensors are powerful platforms for monitoring human and environmental health. Here, we expand their capabilities by interfacing them...
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A target-triggered strand displacement-assisted target recycling based on carbon dots-based fluorescent probe and MSNs@PDA nanoparticles for miRNA amplified detection and fluorescence imaging
A target-triggered strand displacement-assisted target recycling based on carbon dots-based fluorescent probe and mesoporous silica...
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CPA-Cas12a-based lateral flow strip for portable assay of Methicillin-resistant Staphylococcus aureus in clinical sample
The rapid and accurate identification of methicillin-resistant Staphylococcus aureus at an early antibiotic therapy stage would be benefit to disease...
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Rapid and ultrasensitive miRNA detection by combining endonuclease reactions in a rolling circle amplification (RCA)–based hairpin DNA fluorescent assay
MicroRNA (miRNA) sensing strategies employing rolling circle amplification (RCA) coupled with the hairpin DNA (HD) probe–mediated FRET assay have...
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Sensitive naked-eye detection of telomerase activity based on exponential amplification reaction and lateral flow assay
Telomerase is a promising diagnostic and prognostic biomarker for cancers. Sensitive, simple, and reliable telomerase activity detection is vital for...
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SERS immunoassay analysis of Escherichia coli and Staphylococcus aureus based on sandwich-structured complex probe and target-induced strand displacement
A direct and ultra-sensitive surface-enhanced Raman scattering (SERS) immunoassay method is introduced for the detection of Escherichia coli and Staphy...
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Probe-based Loop-Mediated Isothermal Amplification Assay for Multi-target Quantitative Detection of Three Foodborne Pathogens in Seafood
Foodborne pathogens pose serious risks to human health and food safety. More than one pathogen exists in seafood. It is difficult for conventional...
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Cleavable molecular beacon-based loop-mediated isothermal amplification assay for the detection of adulterated chicken in meat
A simple, sensitive, specific and fast method based on the loop-mediated isothermal amplification (LAMP) technique and cleavable molecular beacon...
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Photothermal biosensor for HPV16 based on strand-displacement amplification and gold nanoparticles using a thermometer as readout
Gold nanoparticles (AuNPs) in aggregated state have a strong near infrared region (NIR) absorption and the causes a much stronger photothermal effect...
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A visual denaturation bubble-mediated strand exchange amplification and RGB visual analysis-based assay for quantitative detection of Helicobacter pylori in saliva
Helicobacter pylori ( H. pylori ) is a class I carcinogen causing gastric cancer. Almost 50% of people on earth have been infected and it is worse in...
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Paper-Based Radial Flow Assay Integrated to Portable Isothermal Amplification Chip Platform for Colorimetric Detection of Target DNA
A novel integrated detection system that introduces a paper-chip-based molecular detection strategy into a polydimethylsiloxane (PDMS) microchip and...
