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Chinese Hamster Ovary Cell Line Instability: Causes, Mitigation, and Prediction
Cell line instability is a common problem in biopharmaceutical manufacturing using Chinese hamster ovary (CHO) cells. Cell line instability, which...
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Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of...
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Generation of Monoclonal Chinese Hamster Ovary Cell Lines Using DISPENCELL-S3
Single-cell isolation is a key step in the manufacturing of therapeutic proteins, which relies on the development of monoclonal cell lines. It...
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Protein overproduction alters exosome secretion in Chinese hamster ovary cells
Despite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chinese hamster ovary (CHO)...
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Cell line-specific impact of dexamethasone on the bioprocessing of Chinese hamster ovary cells
This study explored the cell line-specific effects of dexamethasone (DEX) on three antibody-producing Chinese hamster ovary (CHO) clones derived from...
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Limitations of the Plasmid-Based Cas9-Zinc Finger Fusion System for Homology-Directed Knock-In in Chinese Hamster Ovary Cells
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been used for the insertion of...
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Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures
Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various...
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Energy metabolism in Chinese hamster ovary (CHO) cells: Productivity and beyond
Chinese hamster ovary (CHO) cell lines have been widely used to produce recombinant proteins. While the biosynthesis of recombinant proteins is...
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Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
Most pharmaceutical biotechnology companies use stirred-tank bioreactors (STR) for recombinant protein manufacturing. These bioreactors are used at a...
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New high-throughput screening method for Chinese hamster ovary cell lines expressing low reduced monoclonal antibody levels: application of a system controlling the gas phase over cell lysates in miniature bioreactors and facilitating multiple sample setup
Interchain disulfide bonds in monoclonal antibodies may be reduced during large-scale mAb production using Chinese hamster ovary (CHO) cells. This...
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Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter
Although antibodies have attracted attention as next-generation biopharmaceuticals, the costs of purifying the products and of arranging the...
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Transient light-activated gene expression in Chinese hamster ovary cells
BackgroundChinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and...
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Culture media optimization for Chinese hamster ovary cell growth and expression of recombinant varicella-zoster virus glycoprotein E
Herpes zoster (HZ) is caused by reactivation of varicella-zoster virus (VZV) latent in the sensory ganglia and causes severe pain, often leading to...
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Genome-scale modeling of Chinese hamster ovary cells by hybrid semi-parametric flux balance analysis
Flux balance analysis (FBA) is currently the standard method to compute metabolic fluxes in genome-scale networks. Several FBA extensions employing...
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Violacein improves recombinant IgG production by controlling the cell cycle of Chinese hamster ovary cells
Chinese hamster ovary (CHO) cells are used as host cells for industrial monoclonal antibody (mAb) production. Cell cycle control is an effective...
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A case study: Correlation of the nutrient composition in Chinese Hamster Ovary cultures with cell growth, antibody titre and quality attributes using multivariate analyses for guiding medium and feed optimization in early upstream process development
In this case-study, we demonstrate an approach for identifying correlations between nutrients/metabolites in the spent medium of CHO cell cultures...
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Considerations for Glycoprotein Production
This chapter is intended to provide insights for researchers aiming to choose an appropriate expression system for the production of recombinant...
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Determination of Chinese hamster ovary (CHO) cell densities and antibody titers from small volumes of cell culture supernatants using multivariate analysis and partial least squares regression of UV-Vis spectra
Antibody titer and viable cell density (VCD) are two important parameters that need to be closely monitored during the process of cell line...
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Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells
Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a...
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Two-Dimensional Gel Electrophoresis and 2D-DIGE
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the most versatile and widely used techniques to study the...