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Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA
Enzymatic degradation of polyethylene terephthalate (PET) is attracting attention as a new technology because of its mild reaction conditions....
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Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display
Bacterial cis -epoxysuccinic acid hydrolases (CESHs) are intracellular enzymes used in the industrial production of enantiomeric tartaric acids. The...
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Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as...
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Cell-Free Display Techniques for Protein Evolution
Cell-free protein synthesis (CFPS) with flexibility and controllability can provide a powerful platform for high-throughput screening of...
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Streamlining N-terminally anchored yeast surface display via structural insights into S. cerevisiae Pir proteins
Surface display co-opts yeast’s innate ability to embellish its cell wall with mannoproteins, thus converting the yeast’s outer surface into a...
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Adsorption of Lithium on Cell Surface as Nanoparticles through Lithium Binding Peptide Display in Recombinant Escherichia coli
Lithium is one of the highly used elements in various industries such as ceramics, polymer and battery. Considering the enormous demand of lithium...
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Development of a mammalian cell-based ZZ display system for IgG quantification
BackgroundBiological laboratories and companies involved in antibody development need convenient and versatile methods to detect highly active...
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Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
BackgroundMicrobial cell surface display technology allows immobilizing proteins on the cell surface by fusing them to anchoring motifs, thereby...
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Mammalian Antigen Display for Pandemic Countermeasures
Pandemic countermeasures require the rapid design of antigens for vaccines, profiling patient antibody responses, assessing antigen...
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Human super antibody to viral RNA-dependent RNA polymerase produced by a modified Sortase self-cleave-bacteria surface display system
BackgroundRNA-dependent RNA polymerase (RdRp) is a good target of anti-RNA virus agents; not only it is pivotal for the RNA virus replication cycle...
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Display of Lignin Peroxidase on the Surface of Bacillus subtilis
Lignin peroxidase (LiP) has a good application prospect in lignin degradation, environmental treatment, straw feed, and other industries. However,...
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Antigen surface display in two novel whole genome sequenced food grade strains, Lactiplantibacillus pentosus KW1 and KW2
BackgroundUtilization of commensal bacteria for delivery of medicinal proteins, such as vaccine antigens, is an emerging strategy. Here, we describe...
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Display of porcine epidemic diarrhea virus spike protein B-cell linear epitope on Lactobacillus mucosae G01 S-layer surface induce a robust immunogenicity in mice
The Porcine epidemic diarrhea virus (PEDV) presents a substantial risk to the domestic pig industry, resulting in extensive and fatal viral diarrhea...
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A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins
BackgroundNeutralizing antibody plays a key role in protecting hosts from invasive pathogens and their virulent components. Current high-throughput...
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Preparation of Chromobodies for the Detection of Cell Surface Epitopes
Chromobodies are nanobodies genetically fused to fluorescent proteins, which were developed to visualize endogenous intracellular antigens. These...
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Expression of Bispecific Antibodies Using Cellular Display
The identification and selection of high-producing cell lines can be a resource- and time-consuming process. The screening effort can be simplified...
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Whole-cell catalysis by surface display of fluorinase on Escherichia coli using N-terminal domain of ice nucleation protein
BackgroundFluorinases play a unique role in the production of fluorine-containing organic molecules by biological methods. Whole-cell catalysis is a...
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Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation
BackgroundCarbamate pesticides have been widely used in agricultural and forestry pest control. The large-scale use of carbamates has caused severe...
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A Novel Strategy for the Microbial Removal of Heavy Metals: Cell-surface Display of Peptides
Cell surface engineering is a rapidly develo** technology of microorganism that achieves modification of cell surface function by joining external...
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Investigation of nanotopography on SOCE mediated cell migration via live-cell Imaging on opaque implant surface
The exploration of cell response to nanotopography has attracted considerable attentions for years. This article focuses on the influence of...
