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Engineering of a Substrate Affinity Reduced S-Adenosyl-methionine Synthetase as a Novel Biosensor for Growth-Coupling Selection of L-Methionine Overproducers
Biosensors are powerful tools for monitoring specific metabolites or controlling metabolic flux towards the products in a single cell, which play...
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Fundamental Building Blocks of Whole-Cell Biosensor Design
Whole-cell biosensors are one of the most popular applications of synthetic biology. Biological sensing mechanisms originating from natural systems...
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Encoding Genetic Circuits with DNA Barcodes Paves the Way for High-Throughput Profiling of Dose-Response Curves of Metabolite Biosensors
Metabolite biosensors, through which the intracellular metabolite concentrations could be converted to changes in gene expression, are widely used in...
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A new colorimetric lactate biosensor based on CUPRAC reagent using binary enzyme (lactate-pyruvate oxidases)-immobilized silanized magnetite nanoparticles
A novel optical lactate biosensor is presented that utilizes a colorimetric interaction between H 2 O 2 liberated by a binary enzymatic reaction and...
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Novel electrochemical urea biosensor employing gold nanosphere-decorated Prussian blue nanocubes
In this work, a promising, efficient, and sensitive electrochemical biosensor was developed with urease enzyme and a nanointerface consisting of gold...
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Electrochemical biosensor for monitoring fish spoilage based on nanocellulose as enzyme immobilization matrix
Selective and sensitive quantification of xanthine (XA) in food industries has been proved to be highly important for the assessment of the freshness...
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Organic Matter BOD Biosensor Monitoring
From a proposal of a microbial Biochemical Oxygen Demand (BOD) biosensor by Karube et al. in 1977, components (recognition elements, transducer,...
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Pillar[6]arene acts as a biosensor for quantitative detection of a vitamin metabolite in crude biological samples
Metabolic syndrome is associated with obesity, hypertension, and dyslipidemia, and increased cardiovascular risk. Therefore, quick and accurate...
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Biosensor-driven, model-based optimization of the orthogonally expressed naringenin biosynthesis pathway
BackgroundThe rapidly expanding synthetic biology toolbox allows engineers to develop smarter strategies to tackle the optimization of complex...
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Electrochemical and biosensor techniques to monitor neurotransmitter changes with depression
Depression is a common mental illness. However, its current treatments, like selective serotonin reuptake inhibitors (SSRIs) and micro-dosing...
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Quantitative Methods for Metabolite Analysis in Metabolic Engineering
We present a brief overview of metabolic engineering, depicting the necessity of exploiting microorganisms for obtaining desired metabolites and the...
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Fluorescence-Activated Cell Sorting as a Tool for Recombinant Strain Screening
Metabolic engineering of microbial cells is the discipline of optimizing microbial metabolism to enable and improve the production of target...
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Whole Cell Yeast-Based Biosensors
Analyte detection is a major component of fieldwork, environmental surveillance, and health protection, but lack of resources or access to...
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Sensitive Simultaneous Determinations of 1,2-Dihydroxynaphthalene and Catechol by an Amperometric Biosensor
An amperometric biosensor for 1,2-dihydroxynaphthalene (DHN) and catechol (Cat) has been developed in order to monitor the biodegradaton of...
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Polypyrrole/carbon dot nanocomposite as an electrochemical biosensor for liquid biopsy analysis of tryptophan in the human serum of normal and breast cancer women
Liquid biopsy analysis represents a suitable alternative analysis procedure in several cases where no tumor tissue is available or in poor patient...
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Biosensors of the Well-being of Cell Cultures
Genetically-encoded biosensors offer advantages over traditional analytical technologies in biological manufacturing. Various biosensor architectures...
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Live Cell Immobilization
Maintenance of cell viability and physiological cellular activity is crucial in several applications, spanning from microbial fermentation to produce...
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A novel galactose electrochemical biosensor intended for point-of-care measurement of quantitative liver function using galactose single-point test
Liver disease has emerged as a healthcare burden because of high hospitalization rates attributed both to steatohepatitis and to severe hepatic...
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Molecular Mechanism of Enhancement in Basic Tastes by Kokumi Substances: A Potent Calcium-Sensing Receptor (CaSR) Agonist, γ-Glutamyl-Valinyl-Glycine, Amplifies Sweet-Induced ATP Secretion Via Cell-to-Cell Communication in a Mouse Taste Bud
Previously, we reported that agonists for the calcium-sensing receptor CaSR enhance the intensity of salty, sweet, and umami tastes. Interestingly,...
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Cell-Free Biosensors and AI Integration
Cell-free biosensors hold a great potential as alternatives for traditional analytical chemistry methods providing low-cost low-resource measurement...