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Quantitative Methods for Metabolite Analysis in Metabolic Engineering

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Abstract

We present a brief overview of metabolic engineering, depicting the necessity of exploiting microorganisms for obtaining desired metabolites and the difficulty of metabolic pathway optimization under numerous conditions. The advantages, limitations, and examples of conventional quantitative analytical methods that focus on accuracy but have low throughput rates are presented. We have also described in vivo analytical methods with high-throughput rates, which indirectly compare the yield of the reporter protein. Additionally, we have explained a few considerations for engineering in vivo analytical methods. This review also highlights the current challenges faced by the analytical methods in the metabolic engineering.

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Acknowledgements

This work was supported by the Technology Innovation Program [20009356] funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (2022M3A9B6082670).

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  1. Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea

    Cheeyoon Ahn, Min-Kyung Lee & Cheulhee Jung

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Ahn, C., Lee, MK. & Jung, C. Quantitative Methods for Metabolite Analysis in Metabolic Engineering. Biotechnol Bioproc E 28, 949–961 (2023). https://doi.org/10.1007/s12257-022-0200-z

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