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Disposable Bioreactors Used in Process Development and Production Processes with Plant Cell and Tissue Cultures
The bioreactor is the centerpiece of the upstream processing in any biotechnological production process. Its design, the cultivation parameters, the... -
Modeling and Simulation of Cell Cultures in Cultivated Meat Production
The production of cultured meat or in vitro meat is a process developed in several stages, including the proliferation of mother cells and ex situ... -
Evaluation of Antioxidant Activity of Postbiotics in Cell Cultures
The production of intracellular ROS can be measured by using the compound 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), which is a... -
Applications of Fish Cell Cultures
Cell culture serves as a reliable and proficient tool in diverse research fields such as virology, physiology, toxicology, immunology, oncology,... -
Apoptotic effects of Phlomis armeniaca mediated biosynthesized silver nanoparticles in monolayer (2D) and spheroid (3D) cultures of human breast cancer cell lines
The purpose of current research was to assess the apoptotic effects of biofabrication silver nanoparticles (AgNPs) mediated by the aqueous extract of P...
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Biosensors of the Well-being of Cell Cultures
Genetically-encoded biosensors offer advantages over traditional analytical technologies in biological manufacturing. Various biosensor architectures... -
Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures
Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various...
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In Vitro Cultures: Challenges and Limitations
Plant tissue culture provides an effective system for large scale production of plants. Several rare and threatened plants have been conserved... -
Cell Lines for Cultivated Meat Production
Cellular agriculture is an emerging field that aims to provide a more sustainable alternative for the production of animal-derived products, without... -
Cell Suspension Culture-Mediated Secondary Metabolites Production from Medicinal Plants with Antiallergy Properties
The increasing global prevalence of allergic diseases, affecting 20% of the population, necessitates novel antiallergic treatments. Traditional... -
A case study: Correlation of the nutrient composition in Chinese Hamster Ovary cultures with cell growth, antibody titre and quality attributes using multivariate analyses for guiding medium and feed optimization in early upstream process development
In this case-study, we demonstrate an approach for identifying correlations between nutrients/metabolites in the spent medium of CHO cell cultures...
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Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures
BackgroundExtracellular Vesicles (EVs) are sub-micrometer lipid-bound particles released by most cell types. They are considered a promising source...
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Thermoplasmonic Scaffold Design for the Modulation of Neural Activity in Three-Dimensional Neuronal Cultures
Neuromodulation has made great strides in recent years, but in vitro studies have been limited to two-dimensional cell cultures, far from in vivo...
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Introduction to Animal Cell Culture
Animal cell culture has marked a significant revolution in science, allowing different fields to benefit from its use. The aim of this chapter is to... -
Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures
Gazania rigens (L.) Gaertn. (Asteraceae) is a medicinal plant with high ornamental potential and use in landsca**. The therapeutic potential of...
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Utilization of Glucocorticoids as Additives for Enhanced Sialylation of Fc-fusion Protein in CHO Cell Cultures
Sialylation, which is considered as a critical quality attribute, plays important roles in pharmacokinetics of recombinant proteins. Terminal sialic...
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Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures
Chloroplast transformation has been extremely valuable for the study of plastid biology and gene expression, but the tissue culture methodology... -
Biohydrogen Production by Mono- Versus Co- and Mixed Cultures
Acidogenesis and acetogenesis are the key steps in the formation of H2 during the anaerobic decomposition of organic matter. Various groups of... -
Elucidation of the Mechanism and Kinetics of Ultrasonic Extraction of Paclitaxel from Plant Cell Cultures of Taxus chinensis
In this study, the kinetics and mechanism of ultrasonic extraction of paclitaxel from Taxus chinensis were analyzed using ultrasonic cavitation...