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Antibody Purification Using Affinity Chromatography
Antibodies are an integral part of many biological assays and biotherapeutics. However, the sources from which antibodies are derived frequently... -
Mixed-Mode Chromatography and Its Role in Monoclonal Antibody Purification
As the biopharmaceutical industry matures and embraces process intensification methodologies allied to the emergence of newer personalized medicines,... -
Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification
Bispecific antibody (bsAb), a novel therapeutic modality, provides excellent treatment efficacy, yet poses numerous challenges to downstream process...
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Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of... -
Production and Purification of Filovirus Glycoproteins
Ebola (EBOV) and Marburg (MARV) viruses cause hemorrhagic fever disease in humans and non-human primates (NHPs) with case-fatality rates as high as... -
Engineering Homogeneous Photoactive Antibody Fragments
Genetically encoded site-specifically incorporated noncanonical amino acids (ncAAs) have been used to modulate properties of several proteins. Here,... -
Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains
Antibody therapeutics have become a cornerstone of the pharmaceutical market due to their precise molecular targeting, favorable pharmacokinetic... -
Downstream Purification of the Biopharmaceutical Drug Substance
The upstream production process has been designed to yield the intended biopharmaceutical. But the upstream design carries with it a complex impurity... -
A Synopsis of Proteins and Their Purification
The goal of protein purification is to separate a specific protein from all other biomolecules. Classical chromatographic procedures have been... -
Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody
BackgroundCyclin-dependent kinase 4 (CDK4) when hyperactivated drives development and maintenance of most tumour types, thus prompting its use as an...
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Chromatographic Purification of Viral Vectors for Gene Therapy Applications
Chromatography has been a mainstay in the downstream processing and purification of biopharmaceutical medicines. Until now, this has largely involved... -
Magnetic Nanoparticles for Protein Separation and Purification
Proteins are essential for various functions such as brain activity and muscle contraction in humans. Even though food is a source of proteins, the... -
Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Protein fusion technology has had a major impact on the efficient production and purification of individual recombinant proteins. The use of... -
Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines
Capsid virus-like particles (cVLPs), assembled from viral coat proteins, are used as therapeutic cargo delivery vehicles as well as molecular... -
Homogeneous multi-payload antibody–drug conjugates
Many systemic cancer chemotherapies comprise a combination of drugs, yet all clinically used antibody–drug conjugates (ADCs) contain a single-drug...
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Progress of ligand-modified agarose microspheres for protein isolation and purification
Proteins are the material basis of life and the primary carriers of life activities, containing various impurities that must be removed before use....
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Development of a liquid chromatography-based versatile bioanalysis for bevacizumab based on pretreatment combining aptamer affinity purification and centrifugal ultrafiltration concentration
We report on the development of a versatile and accurate bioanalytical method for bevacizumab using a pretreatment method combining affinity...
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Approaches to Avoid Proteolysis During Protein Expression and Purification
All cells contain proteases, which hydrolyze the peptide bonds between amino acids of a protein backbone. Typically, proteases are prevented from... -
Purification of Therapeutic Antibodies by Protein A Affinity Chromatography
Protein A affinity chromatography is widely used for capturing therapeutic antibodies. It offers high binding capacity, selectivity, and resin... -
Human super antibody to viral RNA-dependent RNA polymerase produced by a modified Sortase self-cleave-bacteria surface display system
BackgroundRNA-dependent RNA polymerase (RdRp) is a good target of anti-RNA virus agents; not only it is pivotal for the RNA virus replication cycle...