Search
Search Results
-
Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase
Phthalate acid esters (PAEs), a group of xenobiotic compounds used extensively as plasticizers, have attracted increasing concern for adverse effects...
-
Structural Analysis of Nylon Hydrolase and Enzymatic Approach to Hydrolyze Polyamide Nylon
With the growing social demands for the Sustainable Development Goals (SDGs), there is increasing concern about the environmental pollution of...
-
Fusion proteins of organophosphorus hydrolase and pHluorin for a whole-cell biosensor for organophosphorus pesticide measurement
Fusion proteins composed of an organophosphorus hydrolase (OPH) and pHluorin, a pH-sensitive green fluorescent protein variant, were constructed as...
-
A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases
The quantitation of liberated reducing sugars by the copper-bicinchoninic acid (BCA) assay provides a highly sensitive method for the measurement of...
-
Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression
BackgroundBiocatalysis offers a promising path for plastic waste management and valorization, especially for hydrolysable plastics such as...
-
Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
Use of the 3,5-dinitrosalicylic acid reagent allows the simple, rapid quantification of reducing sugars. The method can be used for analysis of...
-
Synthesis and properties of 1-(3-fluoroadamantan-1-yl)-3-R-ureas and 1,1′-(alkan-1,n-diyl)bis[3-(3-fluoroadamantan-1-yl)ureas], promising inhibitors of epoxide hydrolase sEH
A three-stage method for the preparation of 3-fluoro-1-isocyanatoadamantane in 76% yield was developed using the reaction of...
-
Structural insights into curdlan degradation via a glycoside hydrolase containing a disruptive carbohydrate-binding module
BackgroundDegradation via enzymatic processes for the production of valuable β-1,3-glucooligosaccharides (GOS) from curdlan has attracted...
-
Rational design of GDP‑d‑mannose mannosyl hydrolase for microbial l‑fucose production
Backgroundl ‑Fucose is a rare sugar that has beneficial biological activities, and its industrial production is mainly achieved with brown algae...
-
Transcriptome profiling uncovers the involvement of CmXyn1, a glycosyl hydrolase 11, in Cochliobolus miyabeanus pathogenicity
Necrotrophic pathogen Cochliobolus miyabeanus ( C. miyabeanus ) causes rice brown leaf spot disease and drastically affects the yield and quality of...
-
Engineering Comamonas testosteroni for the production of 2-pyrone-4,6-dicarboxylic acid as a promising building block
BackgroundPlastics are an indispensable part of our daily life. However, mismanagement at their end-of-life results in severe environmental...
-
Degradation of Chlorpyrifos Pesticide Using a Postbiotic Enzyme of Lactic Acid Bacteria
Lactic acid bacteria (LAB) are well known for their production of postbiotic components such as organic acids, short-chain fatty acids, enzymes,...
-
Production of the Minor Ginsenoside F2 from the PPD-mix-type Major Ginsenosides Using a Novel Recombinant Glycoside Hydrolase from Novosphingobium aromaticivorans
BackgroundThe minor ginsenosides such as F 2 , C-K, Rh2, and Rg3 make up less than 1% of the total ginseng extract; however, many studies have shown...
-
Prospects for the inhibition of the phosphatase domain of human soluble epoxide hydrolase (sEH-P)
This brief review highlights the history of the discovery of the phosphatase domain of human soluble epoxide hydrolase (sEH-P). The data on its...
-
Strategies for Monitoring “Ubiquitin C-Terminal Hydrolase 1” (Yuh1) Activity
The family of ubiquitin C-terminal hydrolases (UCHs(releases ε-linked amide bonds positioned at the C-terminus of ubiquitin. UCHL3 is a highly...
-
Identification of novel inhibitors of S-adenosyl-L-homocysteine hydrolase via structure-based virtual screening and molecular dynamics simulations
S -adenosyl- L -homocysteine hydrolase (SAHase) is an important regulator in the methylation reactions in many organisms and thus is crucial for...
-
A Broad-Spectrum α-Glucosidase of Glycoside Hydrolase Family 13 from Marinovum sp., a Member of the Roseobacter Clade
Glycoside hydrolases (GHs) are a diverse group of enzymes that catalyze the hydrolysis of glycosidic bonds. The Carbohydrate-Active enZymes (CAZy)...
-
Functional Immobilization of a Biofilm-Releasing Glycoside Hydrolase Dispersin B on Magnetic Nanoparticles
Dispersin B (DspB) is a member of glycoside hydrolase family 20 (GH20) and catalyzes degradation of biofilms forming by pathogenic bacteria such as Sta...
-
Inhibition of Cyclopropane Fatty Acid Synthesis in the Membrane of Halophilic Halomonas socia CKY01 by Kanamycin
Antibiotics are powerful and reliable substances that can control microorganism growth. However, microbes employ several countermeasures to adapt to...
-
Hydrolysis of lignocellulose to succinic acid: a review of treatment methods and succinic acid applications
Succinic acid (SA) is an intermediate product of the tricarboxylic acid cycle (TCA) and is one of the most significant platform chemicals for the...
