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The use of antifoam agents to eliminate bubbles during biotechnological sample analysis
Colorimetric protein quantitation assays such as the Bradford method are susceptible to foaming when using manual or automated equipment, especially...
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Assessment of thraustochytrids potential for carotenoids, terpenoids and polyunsaturated fatty acids biorefinery
Heterotrophic fast-growing thraustochytrids have been identified as promising candidates for the bioconversion of organic sources into industrially...
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Bio-indigo Production using Wild-type Acinetobacter sp. and Indole-3-acetate Monooxygenase (iacA) Expressed in Escherichia coli
This study reports the identification of a novel bio-indigo-producing bacterium from soil sources. Blue-colored colonies were first screened and...
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Aliivibrio fischeri L-Asparaginase production by engineered Bacillus subtilis: a potential new biopharmaceutical
l -Asparaginase ( l -ASNase) is an enzyme applied in the treatment of lymphoid malignancies. However, an innovative l -ASNase with high yield and lower...
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Development of Responsive Promoters and their Utilization for Stable CHO Sensor Cell Lines
Chinese hamster ovary (CHO) cells are the most important mammalian expression systems to produce recombinant proteins. To ensure a proper expression...
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Proceedings of the 3rd International EUROMAGH Conference 2020 Sustainability and Biobased Materials on the Road of Bioeconomy
This conference proceeding presents selected manuscripts from the EUROMAGH 2020 Conference entitled “Sustainability and Biobased Materials on the...
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CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells
MicroRNAs represent an interesting group of regulatory molecules with the unique ability of a single miRNA able to regulate the expression of...
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Model predictive control for steady-state performance in integrated continuous bioprocesses
Perfusion bioreactors are commonly used for the continuous production of monoclonal antibodies (mAb). One potential benefit of continuous...
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An Overview on Methanotrophs and the Role of Methylosinus trichosporium OB3b for Biotechnological Applications
Rising greenhouse gas concentrations in the atmosphere are having deleterious effects on biotic and abiotic systems, causing serious global concerns....
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Impact of Acetic Acid Supplementation in Polyhydroxyalkanoates Production by Cupriavidus necator Using Mixture-Process Design and Artificial Neural Network
The trend in bioplastic application has increased over the years where polyhydroxyalkanoates (PHAs) have emerged as a potential candidate with the...
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Constitutive expression of nitrilase from Rhodococcus zopfii for efficient biosynthesis of 2-chloronicotinic acid
2-chloronicotinic acid (2-CA) is a key precursor for the synthesis of a series of pesticides and pharmaceuticals. Nitrilase-catalyzed bioprocess is a...
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Improving Fructooligosaccharide Production via sacC Gene Deletion in Zymomonas mobilis: A Novel Approach for Enhanced Prebiotic Production
Fructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. Industrially, these compounds are...
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Versatile Applications of Brewer’s Spent Grain: Solid-State Fermentation and Nutritional Added Value
Brewer’s spent grain (BSG) is a major by-product in the beer-brewing process which contributes to 85% of the entire generated by-product in the...
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Impact of mixing insufficiencies on L-phenylalanine production with an Escherichia coli reporter strain in a novel two-compartment bioreactor
BackgroundThe omnipresence of population heterogeneity in industrial bioprocesses originates from prevailing dynamic bioprocess conditions, which...
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In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis
Bacillus subtilis is a natural producer of 2,3-butanediol (2,3-BDO) and has acquired “Generally Regarded as Safe" status. It is reported to produce...
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Edible mycelium as proliferation and differentiation support for anchorage-dependent animal cells in cultivated meat production
Cultivated meat production requires bioprocess optimization to achieve cell densities that are multiple orders of magnitude higher compared to...
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Luminescence-Based Sensors for Bioprocess Applications
Reliable robust sensor systems are essential to monitor in situ and allow controlling of the evolution of bioprocesses in order to maximize the...
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Whey: As a Fermentation Substrate for the Production of Exopolysaccharides
The dairy and food processing sectors as well as researchers have been attempting to find suitability to utilize by-products of the dairy industry in...
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Metabolic Engineering of Lipid Biosynthesis Pathway to Enhance the Oil Content in Microalgae
Today, a large range of software programmes are used in the field of metabolic engineering. Such applications were shaped to provide support for a...
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Downstream Processing in Marine Biotechnology
Downstream processing is one of the most underestimated steps in bioprocesses and this is not only the case in marine biotechnology. However, it...