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Nylon Monofilament Mold Three-dimensional Microfluidic Chips for Size-exclusion Microchip Electrophoresis: Application to Specific Online Preconcentration of Proteins
We present a lithography-free procedure for fabricating intrinsically three-dimensional microchannels within PDMS elastomers using nylon monofilament...
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A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates
BackgroundProtein nanostructures produced through the self-assembly of individual subunits are attractive scaffolds to attach and position functional...
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Laser scattering centrifugal liquid sedimentation method for the accurate quantitative analysis of mass-based size distributions of colloidal silica
This paper proposes a laser scattering centrifugal liquid sedimentation (LS-CLS) method for the accurate quantitative analysis of the mass-based size...
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Rapid determination of 48 stimulant residues in milk and dairy products by high-performance liquid chromatography coupled with quadrupole/Orbitrap high-resolution mass spectrometry
A fast and easy method was proposed for the determination and quantification of 48 stimulants in milk and dairy products using high-performance...
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Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review
In the recent years, there has been a rapid development of new technologies and strategies when it comes to protein purification and quality control...
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The benefits of mixed-mode chromatography columns for separation of peptides and protein digests
In this work, the evaluation and comparison of mixed-mode chromatography and reversed-phase chromatography for separation of peptides and protein...
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Chromatography of Phenolic Antioxidants
In this chapter, chromatographic techniques widely used for the analysis of phenolic compounds have been reviewed. The concept of chromatography and...
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2D Chromatography and 2D Spectroscopy in Analytical Chemistry: an Overview
AbstractThe development of rapid chromatographic procedures is of utmost relevance to analytical laboratories since the modern pharmaceutical...
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Density-Based Fractionation of Cell-Conditioned Medium to Prepare Proteomics Grade Extracellular Vesicles
The identification of the molecular composition of extracellular vesicles (EV) by omics approaches, including proteomics, requires the separation of...
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Modeling the behavior of monoclonal antibodies on hydrophobic interaction chromatography resins
Monoclonal antibodies (mAbs) require a high level of purity for regulatory approval and safe administration. High-molecular weight (HMW) species are...
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Planar Chromatography as a Method for the Analytical Control of Composite Pharmaceutical Preparations Based on N-Vinylamides
AbstractPlanar chromatography is used to develop methods for the analytical control of processes of the synthesis of biomedical polymers and their...
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Characterization Methods of Amylose and Amylopectin
Amylose and amylopectin are the major constituents of starch that play a significant role in defining the properties of starch and hence its...
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Pushing the limits of size selectivity in nanoscale solute separations
Transport of a spherical solute through a cylindrical pore has been modelled for decades using well-established hindered transport theory, predicting...
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Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry
Lytic polysaccharide monooxygenases perform oxidative cleavage of glycosidic bonds in various polysaccharides. The majority of LMPOs studied so far...
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Design and optimization of a continuous purification process using ion-exchange periodic counter-current chromatography for a low-titer enzyme
A continuous purification process can be beneficial to the purification of biologics due to its higher productivity and efficiency than a...
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Structural identification and absolute quantification of monoclonal antibodies in suspected counterfeits using capillary electrophoresis and liquid chromatography-tandem mass spectrometry
Monoclonal antibodies (mAbs) represent a major category of biopharmaceutical products which due to their success as therapeutics have recently...
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A high-performance liquid chromatography–evaporative light scattering detection–based quantitative analytical method for polyvinyl alcohol in food supplements with pyrolysis–gas chromatography/mass spectrometry-based verification
Polyvinyl alcohol (PVOH) is extensively used in agricultural, pharmaceutical, textile, and food industries. A new high-performance liquid...
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Molecular Structure Characterization of Starch
The physicochemical, functional, and digestibility properties of starch are greatly influenced by the characterization of the molecular structure....
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A correlation of thermodynamic parameters with size of copper-chelated albumin aggregates
Protein conformations are strictly related to its biological function, and the variation in the structure has a major impact on human health. Metal...
