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In Vitro Tube Formation Assays in Matrigel
Vasculature development is a combination of complex processes that require precise coordination of multiple cell types, through time and space, to...
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In Vitro Tumorigenic Assay: A Tumor Sphere Assay for Cancer Stem Cells
Cancer stem cells (CSCs) represent a subpopulation of tumor cells that are thought to be responsible for therapy resistance, recurrence, and...
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Anchorage-Independent Cell Growth Assay for Cancer Stem Cells: Tumorigenic Assay in Vitro
Anchorage-independent growth is a characteristic feature of transformed cells, which means the cells have the ability to grow independently of a...
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A Quantitative Fusion Assay to Study Measles Virus Entry
We have adopted a real-time assay based on a dual-split reporter to assess cell-cell fusion mediated by the measles virus (MeV) membrane fusion...
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Measuring Mutator Enzyme Activity Using an E. coli-Based Colony Formation Assay
Mutator enzymes alter the nucleotide sequences of DNA or RNA molecules; immune systems utilize them to destroy the integrity of pathogen genomes and...
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Cell Viability Assay with 3D Prostate Tumor Spheroids
WST-8 (Cell Counting Kit 8; CCK-8) is the last generation tetrazolium-based cell viability assay and has recently been accepted as a validated method...
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Multiplex Bead Assay for the Serological Surveillance of Measles and Rubella
There is increasing interest in evaluating antibody responses to multiple antigen targets in a single assay. Immunity to measles and rubella are...
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Radiochemical Assay of Monoamine Oxidase Activity
The activity of monoamine oxidase enzymes may be quantified by measuring the conversion of a radiolabeled amine substrate to a radiolabeled product...
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Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay
To integrate with host chromatin and establish a productive infection, HIV-1 must translocate the viral Ribonucleoprotein (RNP) complex through the...
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ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation
Cancer stem cells (CSCs) are a small tumor cell subpopulation, driving cancer initiation, progression, multidrug resistance, and metastasis. Several...
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Quantification of West Nile Virus by Plaque-Forming Assay
The plaque-forming assay is a gold standard technique to determine the concentration of infectious viral particles. In this assay, lytic viruses...
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The microsporidian polar tube: origin, structure, composition, function, and application
Microsporidia are a class of obligate intracellular parasitic unicellular eukaryotes that infect a variety of hosts, even including humans. Although...
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An Overview of Heparin Monitoring with the Anti-Xa Assay
Heparin remains a critical therapy in hospitalized patients requiring anticoagulation. Unfractionated heparin (UFH) mediates its therapeutic effect...
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Micronucleus Assay in Lymphocytes for Human Biomonitoring and Clinical Studies
Micronuclei (MN) are originated when a whole or broken chromosome/chromatin lag at the anaphase of dividing cells and becomes separated from the main...
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Duplex loop-mediated isothermal amplification assay for simultaneous detection of human and human male DNA
ObjectiveScreening of human and human male DNA is necessary for forensic DNA analyses. Although quantitative real-time PCR (qPCR) is commonly used...
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Assessment of Invadopodium Formation and Gelatin Degradation in Vitro
Some cancer cells form highly regulated structures, termed invadopodia, which mediate local, enzymatic degradation of extracellular matrix and...
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Studying Angiogenesis Using Matrigel In Vitro and In Vivo
Angiogenesis plays a critical role in physiology and pathophysiology of the human body; hence, it is important to explore the methods to study...
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Mini-MEndR: a miniaturized 96-well predictive assay to evaluate muscle stem cell-mediated repair
BackgroundFunctional evaluation of molecules that are predicted to promote stem cell mediated endogenous repair often requires in vivo transplant...
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Cell-Based Imaging Assay for Detection of Phospholipidosis
Accumulation of lysosomal phospholipids in cells exposed to cationic amphiphilic drugs is characteristic of drug-induced phospholipidosis. The...
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Identification and functional analysis of rare HECTD1 missense variants in human neural tube defects
Neural tube defects (NTDs) are severe malformations of the central nervous system that arise from failure of neural tube closure. HECTD1 is an E3...
