Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2711)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Vascular Hyperpermeability: Methods and Protocols is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs.
Similar content being viewed by others
Keywords
Table of contents (21 protocols)
-
Front Matter
Editors and Affiliations
Bibliographic Information
Book Title: Vascular Hyperpermeability
Book Subtitle: Methods and Protocols
Editors: Binu Tharakan
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3429-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-3428-8Published: 02 October 2023
Softcover ISBN: 978-1-0716-3431-8Due: 01 November 2023
eBook ISBN: 978-1-0716-3429-5Published: 30 September 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 264
Number of Illustrations: 15 b/w illustrations, 54 illustrations in colour
Topics: Molecular Medicine, Cardiology