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HIV Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Vinayaka R. Prasad

    1. Albert Einstein College of Medicine, Bronx, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Ganjam V. Kalpana

    1. Albert Einstein College of Medicine, Bronx, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2807)

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About this book

This fourth edition volume expands on the previous editions with discussions on the latest methodologies to study HIV, live cell imaging, HIV cure, new modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments. The chapters in this book are organized into seven parts and cover topics such as HIV latency reactivation via single molecule RNA detection; T cell responses; new and efficacious anti-HIV CAR T cells; analysis of mucosal HIV infection; analysis of 3D brain organoids to study neuro AIDS; and the transfer of antibodies across the blood brain barrier. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and authoritative, HIV Protocols, Fourth Edition is a valuable resource for all preclinical HIV-1 researchers looking to learn more about this important and advancing field. 


Keywords

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Visualization of HIV-1 RNA and Proteins

    1. Front Matter

      Pages 1-1
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    2. Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary Cells

      • Stephanie Gambut, Thomas J. Hope, João I. Mamede
      Pages 3-14

    3. Imaging HIV-1 Nuclear Import, Uncoating, and Proviral Transcription

      • Ryan C. Burdick, Alice Duchon, Wei-Shau Hu, Vinay K. Pathak
      Pages 15-30

    4. RNA-FISH for HIV Transcription/Localization Analysis

      • Pavitra Ramdas, Ajit Chande
      Pages 31-43

    5. Single-Cell Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to Visualize the Reactivation of Latent HIV-1

      • Rajiv Pathak, Carolina Eliscovich, Robert H. Singer, Ganjam V. Kalpana
      Pages 45-59

    6. Visualizing HIV-1 Assembly at the T-Cell Plasma Membrane Using Single-Molecule Localization Microscopy

      • Rayane Dibsy, Kaushik Inamdar, Cyril Favard, Delphine Muriaux
      Pages 61-76

    7. Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopy

      • Alice Duchon, Ryan C. Burdick, Vinay K. Pathak, Wei-Shau Hu
      Pages 77-91

    8. Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues

      • Silvana Valdebenito, David Ajasin, Brendan Prideaux, Eliseo A. Eugenin
      Pages 93-110

  3. Biomolecular Condensates

    1. Front Matter

      Pages 111-111
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    2. Characterization of Nuclear HIV-Induced Membraneless Organelles Through Fluorescence Microscopy

      • Viviana Scoca, Francesca Di Nunzio
      Pages 113-125

    3. Detection of CPSF6 in Biomolecular Condensates as a Reporter of HIV-1 Nuclear Import

      • Charlotte Luchsinger, Felipe Diaz-Griffero
      Pages 127-138

  4. HIV-1 Nuclear Import and Chromatin Association

    1. Front Matter

      Pages 139-139
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    2. Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay

      • Adarsh Dharan, Edward M. Campbell
      Pages 141-151

    3. Biochemical Detection of Capsid in the Nucleus During HIV-1 Infection

      • Felipe Diaz-Griffero
      Pages 153-161

    4. Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones and Specific Viral Proteins

      • Franziska K. Geis, Stephen P. Goff
      Pages 163-171

  5. HIV-1 RNA – Functions and Modification

    1. Front Matter

      Pages 173-173
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    2. Measurement of HIV Rev-Rev Response Element Functional Activity

      • Patrick E. H. Jackson, Godfrey Dzhivhuho, **g Huang, Marie-Louise Hammarskjold, David Rekosh
      Pages 175-194

    3. Method for the Enrichment of N6-Methyladenosine-Modified Cellular and HIV-1 RNA

      • Tarun Mishra, Stacia Phillips, Li Wu
      Pages 195-208

    4. Direct Analysis of HIV mRNA m6A Methylation by Nanopore Sequencing

      • Ethan Honeycutt, Fredrick Kizito, Jonathan Karn, Thomas Sweet
      Pages 209-227
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Editors and Affiliations

  • Albert Einstein College of Medicine, Bronx, USA

    Vinayaka R. Prasad, Ganjam V. Kalpana

Bibliographic Information

  • Book Title: HIV Protocols

  • Editors: Vinayaka R. Prasad, Ganjam V. Kalpana

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3862-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3861-3Published: 15 May 2024

  • Softcover ISBN: 978-1-0716-3864-4Due: 15 June 2024

  • eBook ISBN: 978-1-0716-3862-0Published: 14 May 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XIV, 358

  • Number of Illustrations: 3 b/w illustrations, 64 illustrations in colour

  • Topics: Immunology

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