Search
Search Results
-
Optimization of the optical transparency of bones by PACT-based passive tissue clearing
Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological...
-
Non-destructive Characterization of Skeletal Muscle Extracellular Matrix Morphology by Combining Optical Coherence Tomography (OCT) Imaging with Tissue Clearing
Histology is an essential step to visualize and analyze the microstructure of any biological tissue; however, histological processing is often...
-
Affordable optical clearing and immunolabelling in mouse brain slices
Traditional histological analysis is conducted on thin tissue sections, limiting the data capture from large tissue volumes to 2D profiles, and...
-
A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification
Recombinant adeno-associated virus (rAAV) has become one of the most promising gene delivery systems for both in vitro and in vivo applications....
-
Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies
Understanding the inner morphology of intact tissues is one of the most competitive challenges in modern biology. Since the beginning of the...
-
Tissue clearing to examine tumour complexity in three dimensions
The visualization of whole organs and organisms through tissue clearing and fluorescence volumetric imaging has revolutionized the way we look at...
-
Smooth Muscle Organization and Nerves in the Rat Vagina: A First Look Using Tissue Clearing and Immunolabeling
Smooth muscle fibers within the vagina, as well as the nerve fibers that contribute to their control mechanisms, are important for the maintenance...
-
Whole-Brain Clearing and Immunolabelling in the African Killifish Nothobranchius furzeri
While histological sections are the go-to method to analyze spatial information in tissues, three-dimensional information is fundamental to...
-
iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-Throughput Imaging in a Mouse Model of Traumatic Brain Injury
Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896–910,...
-
Computer-assisted three-dimensional quantitation of programmed death-ligand 1 in non-small cell lung cancer using tissue clearing technology
Immune checkpoint blockade therapy has revolutionized non-small cell lung cancer treatment. However, not all patients respond to this therapy....
-
Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo
Tissue optical clearing technique provides a prospective solution for the application of advanced optical methods in life sciences. This chapter...
-
Tissue Clearing and 3-D Visualization of Vasculature with the PEGASOS Method
Tissue clearing techniques turn tissue transparent through a series of chemical and physical treatments. They have provided a useful tool for...
-
Tissue Processing
Tissue processing is the technique by which fixed tissues are made suitable for embedding within a supportive medium such as paraffin, and consists...
-
Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections
Parkinson’s disease (PD) is a neurodegenerative disorder characterized with the progressive loss of dopaminergic (DA) neurons within the substantia...
-
Tissue clearing and its applications in neuroscience
State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire...
-
Role of Inflammation in Tissue Regeneration and Repair
Inflammation plays critical roles in tissue repair and regeneration. After tissue injury, inflammatory immune cells are recruited to the wound site,...
-
Tissue-specific macrophages: how they develop and choreograph tissue biology
Macrophages are innate immune cells that form a 3D network in all our tissues, where they phagocytose dying cells and cell debris, immune complexes,...
-
Techniques in Staining of Rodent and Human Olfactory Tissue
Staining of olfactory tissue allows for evaluation of the organization of specific cell types within the specimen and allows for assessment in...
-
Blood Flow Distribution and Membrane Transporters as Determinant Factors of Tissue Drug Concentration
The free drug hypothesis states that the non-ionized free drug concentrations will have the same value in all the aqueous spaces of the body, once...
-
The Application of 4-Hexylresorcinol in Tissue Engineering
This chapter explores the potential applications of 4-hexylresorcinol (4HR) in tissue engineering, focusing on its use in silk fibroin membrane...