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A single-cell massively parallel reporter assay detects cell-type-specific gene regulation
Massively parallel reporter gene assays are key tools in regulatory genomics but cannot be used to identify cell-type-specific regulatory elements...
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Differential endothelial cell cycle status in postnatal retinal vessels revealed using a novel PIP-FUCCI reporter and zonation analysis
Cell cycle regulation is critical to blood vessel formation and function, but how the endothelial cell cycle integrates with vascular regulation is...
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Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into Mice
Retinopathies marked by photoreceptor cell loss are the main cause of visual impairment and blindness in industrialized societies. Photoreceptor...
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A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro
Hypoxia is a hallmark of ischemic cardiovascular diseases and solid malignant tumors. Cellular hypoxia induces numerous physiological and...
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KappaBle fluorescent reporter mice enable low-background single-cell detection of NF-κB transcriptional activity in vivo
Nuclear factor-κB (NF-κB) is a transcription factor with a key role in a great variety of cellular processes from embryonic development to immunity,...
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Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivo
In vitro studies using cell culture, including three-dimensional cultures without the involvement of tumor vessels, have limitations in simulating...
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The IL-1 promoter-driven luciferase reporter cell line THP-G1b can efficiently predict skin-sensitising chemicals
IL-1 functions as an essential pro-inflammatory mediator for the sensitisation of allergic contact dermatitis (ACD). However, studies conducted to...
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Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate
Wnt/β-catenin signaling plays a major role in regulation of embryogenesis, organogenesis, and adult tissue homeostasis and regeneration. However, the...
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Development of a stable Sf9 insect cell line to produce VSV-G pseudotyped baculoviruses
Baculoviruses have shown great potential as gene delivery vectors in mammals, although their effectiveness in transferring genes varies across...
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Use of CIDEA Reporter Mouse Model for Screening Thermogenic Fat-Activating Drugs
Excessive fat accumulation is a risk factor for metabolic diseases. Activating non-shivering thermogenesis in adipose tissue increases energy...
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The Search for and Functional Analysis of Genetic Variants in microRNA-Binding Sites using Massively Parallel Reporter Assay
A large-scale search for the genetic variants with a bias in the representation of alleles in transcriptome data (AE SNPs) and the binding sites in...
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How to avoid misinterpretation of dual reporter gene assay data affected by cell damage
The activity of nuclear receptors (e.g., pregnane x receptor, PXR) can be assessed by luminescence-based dual reporter gene assays. Under most...
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Detection of CPSF6 in Biomolecular Condensates as a Reporter of HIV-1 Nuclear Import
The initial stages of HIV-1 infection involve the transport of the viral core into the nuclear compartment. The presence of the HIV-1 core in the...
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High-content analysis identified synergistic drug interactions between INK128, an mTOR inhibitor, and HDAC inhibitors in a non-small cell lung cancer cell line
BackgroundThe development of drug resistance is a major cause of cancer therapy failures. To inhibit drug resistance, multiple drugs are often...
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Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand
Molecular forces generated by cell receptors are infrequent and transient, and hence difficult to detect. Here we report an assay that leverages the...
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Gene Editing and Transgenic Reporter Assays in African Killifish
As an emerging genetic model, the short-lived African killifish Nothobranchius furzeri has displayed unique advantages for vertebrate aging and...
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LINE-1 transcription activates long-range gene expression
Long interspersed nuclear element-1 (LINE-1 or L1) is a retrotransposon group that constitutes 17% of the human genome and shows variable expression...
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Screening a small hydrazide-hydrazone combinatorial library for targeting the STAT3 in monocyte-macrophages with insulated reporter transposons
The Signal Transducer and Activator of Transcription 3 (STAT3) pharmacological targeting is regarded as a prospective approach to treat cancer,...
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Cell Lineage Tracing: Colocalization of Cell Lineage Markers with a Fluorescent Reporter
Cell lineage tracing, an old technique which originated in the nineteenth century, regains popularity and relevance due to introduction of a more...
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A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila
Neural activity is modulated over different timescales encompassing subseconds to hours, reflecting changes in external environment, internal state...
