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FLIM-FRET Protein-Protein Interaction Assay
Fluorescence lifetime imaging performed under FRET conditions between two interacting molecules is a sensitive and robust way to quantify... -
Effect of Membrane Permeance and System Parameters on the Removal of Protein-Bound Uremic Toxins in Hemodialysis
Inadequate clearance of protein-bound uremic toxins (PBUTs) during dialysis is associated with morbidities in chronic kidney disease patients. The...
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A Simple and Low-cost Preliminary Quantification of Target Membrane Protein in Single Cells
To study the heterogeneity of target membrane proteins in single cells with cellular integrity, we proposed a simple and low-cost method to obtain...
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Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma
O-Glycan synthesis enzyme glucosaminyl (N-acetyl) transferase 3 (GCNT3) is closely related to the occurrence and development of various...
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Membrane protein trafficking in the anti-tumor immune response: work of endosomal-lysosomal system
Immunotherapy has changed the treatment landscape for multiple cancer types. In the recent decade, great progress has been made in immunotherapy,...
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CCAAT enhancer binding protein delta activates vesicle associated membrane protein 3 transcription to enhance chemoresistance and extracellular PD-L1 expression in triple-negative breast cancer
BackgroundChemoresistance and immunosuppression are two major obstacles in the current anti-cancer treatments. This study investigates the...
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Altered vacuole membrane protein 1 (VMP1) expression is associated with increased NLRP3 inflammasome activation and mitochondrial dysfunction
BackgroundAltered expression of vacuole membrane protein 1 (VMP1) has recently been observed in the context of multiple sclerosis and Parkinson’s...
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Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System
Antibodies specifically recognizing integral membrane proteins are essential tools for functional analysis, diagnosis, and therapeutics targeting... -
The vertebrate- and testis- specific transmembrane protein C11ORF94 plays a critical role in sperm-oocyte membrane binding
Sperm-oocyte membrane fusion is necessary for mammalian fertilization. The factors that determine the fusion of sperm with oocytes are largely...
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Reconstruction of Protein/Liposome Complex
Most ion channels and receptors are distributed in cell membranes and are known as membrane proteins. These membrane proteins are folded in the cell... -
Protein Structure and Modification of Aquaporins
Aquaporins (AQPs) allow water molecules and other small, neutral solutes to quickly pass through membrane. The protein structures of AQPs solved by... -
Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar glomerulus
We previously reported that the membrane skeletal protein 4.1G in the peripheral nervous system transports membrane palmitoylated protein 6 (MPP6),...
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Co-immunoprecipitation Assays to Detect Protein–Protein Interactions
Proteins usually do not function as monomers but rather perform their functions by interacting with themselves or other proteins.... -
Akkermansia muciniphila and its membrane protein ameliorates intestinal inflammatory stress and promotes epithelial wound healing via CREBH and miR-143/145
BackgroundThe intestinal epithelial barrier is the interface for interaction between gut microbiota and host metabolic systems. Akkermansia...
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Reduced polymorphism of Plasmodium vivax early transcribed membrane protein (PvETRAMP) 11.2
BackgroundETRAMP11.2 (PVX_003565) is a well-characterized protein with antigenic potential. It is considered to be a serological marker for...
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Identification of Timm13 protein translocase of the mitochondrial inner membrane as a potential mediator of liver fibrosis based on bioinformatics and experimental verification
ObjectiveTo explore the association between translocase of the inner mitochondrial membrane 13 (Timm13) and liver fibrosis.
MethodsGene expression...
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High GLUT1 membrane expression and low PSMA membrane expression in Ductal Adenocarcinoma and Intraductal Carcinoma of the prostate
BackgroundBoth Ductal Adenocarcinoma (DAC) and Intraductal Carcinoma (IDC) of the prostate are generally associated with aggressive clinical behavior...
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The Split-Luciferase Complementation Assay to Detect and Quantify Protein–Protein Interactions in Planta
Protein–protein interactions play a critical role in plant viral infection and defense responses against pathogens. This protocol provides a detailed... -
Two peptides derivate from Acinetobacter baumannii outer membrane protein K as vaccine candidates: a comprehensive in silico study
BackgroundThe lack of appropriate vaccines is an obstacle to the effective management of A. baumannii infections. Peptide vaccines offer an...
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The Presynaptic α2δ Protein Family and Their Therapeutic Potential
α2δ proteins are well-established modulators of membrane expression and biophysical properties of voltage-gated calcium channels. Moreover, they are...