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Emerging Human Pluripotent Stem Cell-Based Human–Animal Brain Chimeras for Advancing Disease Modeling and Cell Therapy for Neurological Disorders
Human pluripotent stem cell (hPSC) models provide unprecedented opportunities to study human neurological disorders by recapitulating human-specific...
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High-dimensional single-cell analysis of human natural killer cell heterogeneity
Natural killer (NK) cells are innate lymphoid cells (ILCs) contributing to immune responses to microbes and tumors. Historically, their...
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Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis
Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T...
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Single-cell analysis of human MAIT cell transcriptional, functional and clonal diversity
Mucosal-associated invariant T (MAIT) cells are innate-like T cells that recognize microbial metabolites through a semi-invariant T cell receptor...
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Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells
Whole cell patch clamp recording techniques are commonly used to assay membrane excitability, ion channel function, and synaptic activity in neurons....
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Identification and Purification of Human T Cell Precursors
During their development, human T cells undergo similar genomic changes and pass through the same developmental checkpoints as develo** thymocytes...
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Single-cell epigenomic reconstruction of developmental trajectories from pluripotency in human neural organoid systems
Cell fate progression of pluripotent progenitors is strictly regulated, resulting in high human cell diversity. Epigenetic modifications also...
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Single-cell genomics meets human genetics
Single-cell genomic technologies are revealing the cellular composition, identities and states in tissues at unprecedented resolution. They have now...
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In Vitro Model Systems to Study Human T Cell Development
Not only is human T cell development characterized by unique changes in surface marker expression, but it also requires specific growth factors and...
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Integrating population genetics, stem cell biology and cellular genomics to study complex human diseases
Human pluripotent stem (hPS) cells can, in theory, be differentiated into any cell type, making them a powerful in vitro model for human biology....
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A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs...
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Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation
Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune...
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Analysis of connexin 43, connexin 45 and N-cadherin in the human sertoli cell line FS1 and the human seminoma-like cell line TCam-2 in comparison with human testicular biopsies
BackgroundGerm cell tumors are relatively common in young men. They derive from a non-invasive precursor, called germ cell neoplasia in situ, but the...
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CRISPR-Cas9 screens reveal common essential miRNAs in human cancer cell lines
BackgroundGenome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies...
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A single-cell transcriptome atlas of human euploid and aneuploid blastocysts
Aneuploidy is frequently detected in early human embryos as a major cause of early pregnancy failure. However, how aneuploidy affects cellular...
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Human amniotic epithelial stem cell is a cell therapy candidate for preventing acute graft-versus-host disease
Graft-versus-host disease (GVHD), an immunological disorder that arises from donor T cell activation through recognition of host alloantigens, is the...
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Identification of three distinct cell populations for urate excretion in human kidneys
In humans, uric acid is an end-product of purine metabolism. Urate excretion from the human kidney is tightly regulated by reabsorption and...
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Single-cell transcriptomic landscape of the develo** human spinal cord
Understanding spinal cord assembly is essential to elucidate how motor behavior is controlled and how disorders arise. The human spinal cord is...
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Boosting cytotoxicity of adoptive allogeneic NK cell therapy with an oncolytic adenovirus encoding a human vIL-2 cytokine for the treatment of human ovarian cancer
Despite good results in the treatment of hematological malignancies, Natural killer (NK) cells have shown limited effectiveness in solid tumors, such...
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Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines
For nearly a generation now, OP9-DL1 and OP9-DL4 cells have provided an efficient and reliable cell system to generate T cells from mouse and human...