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Considerations for the optimization of in vitro models of chloropicrin toxicity
Chloropicrin (CP) is a common agricultural fumigant historically used as a chemical warfare agent and is a concern for potential use in warfare and...
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Biofabrication, biochemical profiling, and in vitro applications of salivary gland decellularized matrices via magnetic bioassembly platforms
Trending three-dimensional tissue engineering platforms developed via biofabrication and bioprinting of exocrine glands are on the rise due to a...
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Measurements of transepithelial electrical resistance (TEER) are affected by junctional length in immature epithelial monolayers
The measurement of transepithelial electrical resistance (TEER) is a common technique to determine the barrier integrity of epithelial cell...
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Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins
Many negative-sense single-stranded RNA viruses within the order Mononegavirales harm humans. A common feature shared among cells infected by these...
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In Vivo Modeling of Leukemia by Murine Stem Cell Transplantation
Murine stem cell transplantation is a well-established method for the in vivo study of leukemic pathophysiology. Adoptive transfer of murine leukemic...
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Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interface
The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary...
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Yap governs a lineage-specific neuregulin1 pathway-driven adaptive resistance to RAF kinase inhibitors
BackgroundInactivation of the Hippo pathway promotes Yap nuclear translocation, enabling execution of a transcriptional program that induces tissue...
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Creation of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel Derived from a Size-Sorted Diploid Clone
Cell line panels have proven to be an invaluable tool for investigators researching a range of topics from drug mechanism or drug sensitivity studies...
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Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environment
The human lung adenocarcinoma cell line A549 is commonly used in cancer research as a model of malignant alveolar type II epithelial cells. A549...
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Evaluating Chromosome Instability and Genotoxicity Through Single Cell Quantitative Imaging Microscopy
Across eukaryotes, genome stability is essential for normal cell function, physiology, and species survival. Aberrant expression of key genes or...
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Monitoring Cell Cytoskeleton Variations upon Piezoelectric Stimulation: Implications for the Immune System
Piezoelectric stimulation can have a significant impact on different cellular functions with possible applications in several fields, such as...
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Reverse Genetics System for Rift Valley Fever Virus
Rift Valley fever virus (RVFV) is an important mosquito-borne virus that can cause severe disease manifestations in humans including ocular damage,...
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Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection
Measles virus (MeV) infection of airway surface epithelial cells provides a site for final amplification before being released back into the...
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Quantification of West Nile Virus by Plaque-Forming Assay
The plaque-forming assay is a gold standard technique to determine the concentration of infectious viral particles. In this assay, lytic viruses...
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Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors
The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological...
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Primary Intestinal Fibroblasts: Isolation, Cultivation, and Maintenance
Intestinal fibroblasts maintain homeostasis and contribute to inflammatory responses and the development of cancer. Intestinal fibroblasts express...
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Optical Control of Mononegavirus Gene Expression and Replication
Mononegaviruses are promising tools as oncolytic and transgene vectors for gene therapy and regenerative medicine. However, when mononegaviruses are...
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In Vitro Experimental Approach for Studying Human Pulmonary Artery Smooth Muscle Cells and Endothelial Cells Proliferation and Migration
Pulmonary arterial hypertension (PAH) is a severe vascular disease characterized by persistent precapillary pulmonary hypertension, leading to right...
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Studying Monocyte-Myofibroblast Immunomodulation in a 3D Co-culture System
Simple and reproducible 3D cell culture systems that mimic biological interactions within physiological tissues (biomimetics) can provide unique...
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Generation and Co-culture of Cortical Glutamatergic and GABAergic-Induced Neuronal Cells
The study of neurological disorders requires experimentation on human neurons throughout their development. Primary neurons can be difficult to...