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A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses
The baculovirus expression vector system (BEVS) is recognized as a powerful platform for producing challenging proteins and multiprotein complexes...
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Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System
The baculovirus expression vector system (BEVS) has now found acceptance in both research laboratories and industry, which can be attributed to many...
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TOPO Cloning for Efficient Plasmid Construction
This chapter outlines the use of TOPO cloning for streamlined generation of a recombinant plasmid containing your gene of interest for use in the...
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Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System
Insect cell expression has been successfully used for the production of viral antigens as part of commercial vaccine development. As expression host,...
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Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System
Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns....
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Functional Baculovirus Particle Quantification via Plaque Assay
Plaque assay method enables the quantification of infectious baculovirus when defined as plaque forming units (PFU). It allows to determine the...
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Expression and Purification of Inflammasome Sensor NOD-Like Receptor Protein-1 Using the Baculovirus-Insect Cell Expression System
Inflammasomes are innate immune sensing and signaling complexes critical for defense against pathogens and response to cellular stresses. A core...
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Recombinant AAV Production
The insect cell-baculovirus expression vector (IC-BEV) platform has enabled small research-scale and large commercial-scale production of recombinant...
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Scarless Baculovirus Genome Editing Using Lambda-Red Recombineering in E. coli
Baculoviruses are widely used for their potential as biological pesticide and as platform for the production of recombinant proteins and gene therapy...
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Assembly of Baculovirus Vectors for Multiplexed Prime Editing
Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to...
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Construction and Characterization of a Novel Bacmid AcBac-Syn Based on a Synthesized Baculovirus Genome
Baculoviruses are large DNA viruses which have been widely used as expression vectors and biological insecticides. Homologous recombination and...
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TCID50 Assay: A Simple Method to Determine Baculovirus Titer
There are many methods that can be used to determine the infectious titer of your baculovirus stock. The TCID50 method is a simple end-point dilution...
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Stability and integrity of self-assembled bovine parvovirus virus‑like particles (BPV‑VLPs) of VP2 and combination of VP1VP2 assisted by baculovirus-insect cell expression: a potential logistical platform for vaccine deployment
BackgroundBovine parvovirus (BPV) is an autonomous DNA virus with a smaller molecular size and subtle differences in its structural proteins, unlike...
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BacMam virus-based surface display for HCV E2 glycoprotein induces strong cross-neutralizing antibodies and cellular immune responses in vaccinated mice
BackgroundDespite recent advancements, limitations in the treatment and control of hepatitis C virus (HCV) infection reprioritized the studies for...
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Construction of Recombinant Baculoviruses
The success of using the insect cell-baculovirus expression technology (BEST) relies on the efficient construction of recombinant baculovirus with...
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Purification Process for a Secreted Protein Produced in Insect Cells
The Baculovirus Expression Vector System (BEVS) is used with cultured insect cells to produce a wide variety of heterologous proteins, which can be...
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Easily purified baculovirus/insect-system-expressed recombinant hepatitis B virus surface antigen fused to the N- or C-terminus of polyhedrin
Baculoviruses are circular double-stranded DNA viruses that infect insects and are widely used as the baculoviral expression vectors (BEVs), which...
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Optimized Workflow from Gene to Product
This chapter outlines the workflow using the ExpiSf™ Expression System designed for high-density infection of suspension ExpiSf9™ cells. The system...
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Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system
Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical...
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Efficient application of a baculovirus-silkworm larvae expression system for obtaining porcine circovirus type 2 virus-like particles for a vaccine
Porcine circovirus type 2 (PCV2) is a major pathogen associated with swine diseases. It is the smallest single-stranded DNA virus, and its genome...
