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  1. Article

    Open Access

    RAISING is a high-performance method for identifying random transgene integration sites

    Both natural viral infections and therapeutic interventions using viral vectors pose significant risks of malignant transformation. Monitoring for clonal expansion of infected cells is important for detecting ...

    Yusaku Wada, Tomoo Sato, Hiroo Hasegawa, Takahiro Matsudaira in Communications Biology (2022)

  2. Article

    Open Access

    Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

    Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of C...

    Tomomi Aida, Keiho Chiyo, Takako Usami, Harumi Ishikubo, Risa Imahashi in Genome Biology (2015)

  3. Article

    Open Access

    Development of detection method for novel fusion gene using GeneChip exon array

    Fusion genes have been recognized to play key roles in oncogenesis. Though, many techniques have been developed for genome-wide analysis of fusion genes, a more efficient method is desired.

    Yusaku Wada, Masaaki Matsuura, Minoru Sugawara in Journal of Clinical Bioinformatics (2014)