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  1. Article

    “De-novo” amino acid sequence elucidation of protein G′e by combined “Top-Down” and “Bottom-Up” mass spectrometry

    Mass spectrometric de-novo sequencing was applied to review the amino acid sequence of a commercially available recombinant protein G´ with great scientific and economic importance. Substantial deviations to t...

    Yelena Yefremova, Mahmoud Al-Majdoub… in Journal of The American Society for Mass S… (2015)

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    Fast Photochemical Oxidation of Proteins (FPOP) Maps the Epitope of EGFR Binding to Adnectin

    Epitope map** is an important tool for the development of monoclonal antibodies, mAbs, as therapeutic drugs. Recently, a class of therapeutic mAb alternatives, adnectins, has been developed as targeted biolo...

    Yuetian Yan, Guodong Chen, Hui Wei… in Journal of The American Society for Mass S… (2014)

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    High-Energy Collision-Induced Dissociation by MALDI TOF/TOF Causes Charge-Remote Fragmentation of Steroid Sulfates

    A method for structural elucidation of biomolecules dating to the 1980s utilized high-energy collisions (~10 keV, laboratory frame) that induced charge-remote fragmentations (CRF), a class of fragmentations pa...

    Yuetian Yan, Masaaki Ubukata, Robert B. Cody… in Journal of The American Society for Mass S… (2014)

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    Mass Spectrometry Combinations for Structural Characterization of Sulfated-Steroid Metabolites

    Steroid conjugates, which often occur as metabolites, are challenging to characterize. One application is female-mouse urine, where steroid conjugates serve as important ligands for the pheromone-sensing neuro...

    Yuetian Yan, Don L. Rempel, Timothy E. Holy… in Journal of The American Society for Mass S… (2014)