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  1. No Access

    Article

    Metabolic engineering and optimization of the fermentation medium for vitamin B12 production in Escherichia coli

    Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artific...

    Dong Li, Huan Fang, Yuanming Gai, **g Zhao… in Bioprocess and Biosystems Engineering (2020)

  2. Article

    Open Access

    A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker

    Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expres...

    **gqi Chen, Zhaoxia **, Yuanming Gai, Jibin Sun… in Bioresources and Bioprocessing (2017)

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  3. Article

    Open Access

    Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression

    Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, produ...

    **gqi Chen, Gang Fu, Yuanming Gai, ** Zheng, Dawei Zhang… in Microbial Cell Factories (2015)

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