15 Result(s)
within
Yoshitaka Shirasaki
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Article
Open AccessTime-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation
The decision of whether cells are activated or not is controlled through dynamic intracellular molecular networks. However, the low population of cells during the transition state of activation renders the ana...
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Article
Open AccessWhole-embryonic identification of maternal microchimeric cell types in mouse using single-cell RNA sequencing
Even though the mother and the fetus of placental mammals are immunologically non-self with respect to one other, mutual exchange of small numbers of cells between them is known to occur. Maternal cells enteri...
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Protocol
Time-Lapse Imaging of Necroptosis and DAMP Release at Single-Cell Resolution
Necroptosis is a regulated form of necrosis that depends on receptor-interacting protein kinase (RIPK)3 and mixed lineage kinase domain-like protein (MLKL). Necroptotic cells release a variety of cellular and ...
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Protocol
Live-Cell Imaging Technique to Visualize DAMPs Release During Regulated Cell Death
The present protocol introduces a live-cell imaging of secretion activity (LCI-S) that is useful to visualize the real-time release of molecules from individual cells using an immunoassay coupled with total in...
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Article
Open AccessRaman image-activated cell sorting
The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent...
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Article
Author Correction: A practical guide to intelligent image-activated cell sorting
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
A practical guide to intelligent image-activated cell sorting
Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that performs real-time intelligent image-based sorting of single cells with high throughput. iIACS extends beyond the capa...
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Article
A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain
Deficiency in the deubiquitinating enzyme A20 causes severe inflammation in mice, and impaired A20 function is associated with human inflammatory diseases. A20 has been implicated in negatively regulating NF-κ...
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Article
Open AccessAddendum: A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs
The cDNA sequence of human SMART described in this Article was misreported, as described in the accompanying Addendum. This error does not affect the results or any conclusion of the Article.
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Article
Open AccessA FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs
Necroptosis is a regulated form of necrosis that depends on receptor-interacting protein kinase (RIPK)3 and mixed lineage kinase domain-like (MLKL). While danger-associated molecular pattern (DAMP)s are involv...
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Protocol
Challenges in Develo** Protein Secretion Assays at a Single-Cell Level
In addition to direct physical interactions between/among cells, the secretion of humoral factors from living cells is a critical process for cell-cell communications. A well-known extracellular signaling even...
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Article
Open AccessConstruction of a system using a deep learning algorithm to count cell numbers in nanoliter wells for viable single-cell experiments
For single-cell experiments, it is important to accurately count the number of viable cells in a nanoliter well. We used a deep learning-based convolutional neural network (CNN) on a large amount of digital da...
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Article
Open AccessReal-time single-cell imaging of protein secretion
Protein secretion, a key intercellular event for transducing cellular signals, is thought to be strictly regulated. However, secretion dynamics at the single-cell level have not yet been clarified because inte...
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Protocol
Single-Molecule Tracking of mRNA in Living Cells
Some mRNAs localize to specific regions within eukaryotic cells to express their functions. The movement and localization of mRNA molecules provides valuable information about how they concentrate to particula...
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Chapter and Conference Paper
A Novel Biomolecule Sorter Using Thermosensitve Hydrogel in Micro Flow System
A novel biomolecule sorter using thermoreversible hydrogel was developed. Thermosensitive block copolymer changes its state from sol to gel when it is heated. The solution containing fluorescent biomolecules a...
