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Article
Open AccessNSUN3-mediated mitochondrial tRNA 5-formylcytidine modification is essential for embryonic development and respiratory complexes in mice
In mammalian mitochondria, translation of the AUA codon is supported by 5-formylcytidine (f5C) modification in the mitochondrial methionine tRNA anticodon. The 5-formylation is initiated by NSUN3 methylase. Human...
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Article
Open AccessResistance to chemical carcinogenesis induction via a dampened inflammatory response in naked mole-rats
Naked mole-rats (NMRs) have a very low spontaneous carcinogenesis rate, which has prompted studies on the responsible mechanisms to provide clues for human cancer prevention. However, it remains unknown whethe...
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Article
Open AccessIsolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
The naked mole-rat (NMR) is the longest-lived rodent with a maximum lifespan of more than 37 years and shows a negligible senescence phenotype, suggesting that tissue stem cells of NMRs are highly capable of m...
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Article
Open AccessCharacterization of an active LINE-1 in the naked mole-rat genome
Naked mole-rats (NMRs, Heterocephalus glaber) are the longest-living rodent species. A reason for their long lifespan is pronounced cancer resistance. Therefore, researchers believe that NMRs have unknown secrets...
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Chapter
Induced Pluripotent Stem Cells from Cancer-Resistant Naked Mole-Rats
Stem cells play essential roles in the development and tissue homeostasis of animals and are closely associated with carcinogenesis and aging. Also, the somatic cell reprogramming process to induced pluripoten...
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Article
Open AccessCharacterization of brown adipose tissue thermogenesis in the naked mole-rat (Heterocephalus glaber), a heterothermic mammal
The naked mole-rat (NMR) is a heterothermic mammal that forms eusocial colonies consisting of one reproductive female (queen), several reproductive males, and subordinates. Despite their heterothermy, NMRs pos...
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Article
Open AccessFlow cytometric identification and cell-line establishment of macrophages in naked mole-rats
Naked mole rats (NMRs) have extraordinarily long lifespans and anti-tumorigenic capability. Recent studies of humans and mice have shown that many age-related diseases, including cancer, are strongly correlate...
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Article
Open AccessProtocadherin-αC2 is required for diffuse projections of serotonergic axons
Serotonergic axons extend diffuse projections throughout various brain areas, and serotonergic system disruption causes neuropsychiatric diseases. Loss of the cytoplasmic region of protocadherin-α (Pcdh-α) fam...
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Article
Open AccessTumour resistance in induced pluripotent stem cells derived from naked mole-rats
The naked mole-rat (NMR, Heterocephalus glaber), which is the longest-lived rodent species, exhibits extraordinary resistance to cancer. Here we report that NMR somatic cells exhibit a unique tumour-suppressor re...
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Protocol
Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species
Pluripotent stem cells can provide us with an enormous cell source for in vitro model systems for development. In 2006, new methodology was designed to generate pluripotent stem cells directly from somatic cel...
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Article
Split single-cell RT-PCR analysis of Purkinje cells
This protocol details a method for analyzing the expression of multiple genes from a single Purkinje neuron, including the determination of whether the gene expression is monoallelic or biallelic. The protocol...
