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Open AccessTools for microbial single-cell genomics for obtaining uncultured microbial genomes
The advent of next-generation sequencing technologies has facilitated the acquisition of large amounts of DNA sequence data at a relatively low cost, leading to numerous breakthroughs in decoding microbial gen...
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Open AccessTargeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral
Endozoicomonas bacteria symbiosis with various marine organisms is hypothesized as a potential indicator of health in corals. Although many amplicon analyses using 16S rRNA gene have suggested the diversity of En...
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Correction: The Effect of Co-Culture of Two Coral Species on Their Bacterial Composition Under Captive Environments
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Open AccessStrain-level profiling of viable microbial community by selective single-cell genome sequencing
Culture-independent analysis with high-throughput sequencing has been widely used to characterize bacterial communities. However, signals derived from non-viable bacteria and non-cell DNA may inhibit its chara...
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MBC2019: Marine Biotechnology Conference 2019
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Open AccessSingle-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota
The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabol...
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Open AccessHigh-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functio...
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Open AccessCorrection to: A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro
After publication of the original article [1], the authors became aware of a miscalculation in the original Fig. 2d.
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Open AccessA CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro
Resting CD4+ T cells are major reservoirs of latent HIV-1 infection, and may be formed during the early phase of the infection. Although CCR5-tropic (R5) HIV-1 is highly transmissible during the early phase, newl...
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Open AccessObtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes
Single-cell genomics is a straightforward approach to obtain genomes from uncultured microbes. However, sequence reads from a single-cell amplified genome (SAG) contain significant bias and chimeric sequences....
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Open AccessMassively parallel whole genome amplification for single-cell sequencing using droplet microfluidics
Massively parallel single-cell genome sequencing is required to further understand genetic diversities in complex biological systems. Whole genome amplification (WGA) is the first step for single-cell sequenci...