13 Result(s)

Article

Tools for microbial single-cell genomics for obtaining uncultured microbial genomes

The advent of next-generation sequencing technologies has facilitated the acquisition of large amounts of DNA sequence data at a relatively low cost, leading to numerous breakthroughs in decoding microbial gen...

Masahito Hosokawa, Yohei Nishikawa in Biophysical Reviews (2024)

Article

Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral

Endozoicomonas bacteria symbiosis with various marine organisms is hypothesized as a potential indicator of health in corals. Although many amplicon analyses using 16S rRNA gene have suggested the diversity of En...

Keigo Ide, Yohei Nishikawa, Toru Maruyama, Yuko Tsukada, Masato Kogawa… in Microbiome (2022)

Article

Correction: The Effect of Co-Culture of Two Coral Species on Their Bacterial Composition Under Captive Environments

Keigo Ide, Yoshikatsu Nakano, Michihiro Ito, Yohei Nishikawa… in Marine Biotechnology (2022)

Article

Strain-level profiling of viable microbial community by selective single-cell genome sequencing

Culture-independent analysis with high-throughput sequencing has been widely used to characterize bacterial communities. However, signals derived from non-viable bacteria and non-cell DNA may inhibit its chara...

Masahito Hosokawa, Taruho Endoh, Kazuma Kamata, Koji Arikawa… in Scientific Reports (2022)

Article

MBC2019: Marine Biotechnology Conference 2019

Haruko Takeyama, Hiroshi Saito, Yohei Nishikawa in Marine Biotechnology (2020)

Article

Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota

The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabol...

Rieka Chijiiwa, Masahito Hosokawa, Masato Kogawa, Yohei Nishikawa, Keigo Ide… in Microbiome (2020)

Article

High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics

Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functio...

Kenshi Yaginuma, Wataru Aoki, Natsuko Miura, Yuta Ohtani… in Scientific Reports (2019)

Article

Correction to: A CCR5⁺ memory subset within HIV-1-infected primary resting CD4⁺ T cells is permissive for replication-competent, latently infected viruses in vitro

After publication of the original article [1], the authors became aware of a miscalculation in the original Fig. 2d.

Kazutaka Terahara, Ryutaro Iwabuchi, Masahito Hosokawa… in BMC Research Notes (2019)

Article

A CCR5⁺ memory subset within HIV-1-infected primary resting CD4⁺ T cells is permissive for replication-competent, latently infected viruses in vitro

Resting CD4⁺ T cells are major reservoirs of latent HIV-1 infection, and may be formed during the early phase of the infection. Although CCR5-tropic (R5) HIV-1 is highly transmissible during the early phase, newl...

Kazutaka Terahara, Ryutaro Iwabuchi, Masahito Hosokawa… in BMC Research Notes (2019)

Article

Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes

Single-cell genomics is a straightforward approach to obtain genomes from uncultured microbes. However, sequence reads from a single-cell amplified genome (SAG) contain significant bias and chimeric sequences....

Masato Kogawa, Masahito Hosokawa, Yohei Nishikawa, Kazuki Mori… in Scientific Reports (2018)

Article

Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics

Massively parallel single-cell genome sequencing is required to further understand genetic diversities in complex biological systems. Whole genome amplification (WGA) is the first step for single-cell sequenci...

Masahito Hosokawa, Yohei Nishikawa, Masato Kogawa, Haruko Takeyama in Scientific Reports (2017)