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Article
Open AccessPeptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies
Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated indiv...
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Article
Specific Detection of Coral-Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater
Coral microbial flora has been attracting attention because of their potential to protect corals from environmental stresses or pathogens. Although coral-associated bacteria are considered to be acquired from ...
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Article
Open AccessHigh-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functio...
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Article
Open AccessDomain swap** of complementarity-determining region in nanobodies produced by Pichia pastoris
Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies,...
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Article
Ruegeria sp. Strains Isolated from the Reef-Building Coral Galaxea fascicularis Inhibit Growth of the Temperature-Dependent Pathogen Vibrio coralliilyticus
The coral microbiome has attracted increased attention because of its potential roles in host protection against deadly diseases. However, little is known about the role of coral-associated bacteria against th...
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Chapter
Enzyme Evolution
In this chapter, we discuss how to obtain enzymes with desired functions by using yeast cell surface display methods. Although useful enzymes can be found in natural environments and in databases, catalytic ac...
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Article
Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles
Coral reefs are one of the most biologically diverse and economically important ecosystems on earth. However, the destruction of coral reefs has been reported worldwide owing to rising seawater temperature ass...
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Article
Open AccessReconstruction of thermotolerant yeast by one-point mutation identified through whole-genome analyses of adaptively-evolved strains
Saccharomyces cerevisiae is used as a host strain in bioproduction, because of its rapid growth, ease of genetic manipulation, and high reducing capacity. However, the heat produced during the fermentation proces...
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Protocol
Enzyme Evolution by Yeast Cell Surface Engineering
Artificial evolution of proteins with the aim of acquiring novel or improved functionality is important for practical applications of the proteins. We have developed yeast cell surface engineering methods (or ...
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Protocol
Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae
Recent development of large-scale analyses such as the secretome analysis has enabled the discovery of a vast number of intracellular proteins that are secreted outside the cell. Often, these proteins do not c...
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Article
Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering
Pyrosequencing system utilizing luciferase is one of the next-generation DNA sequencing systems. However, there is a crucial problem with the current pyrosequencing system: luciferase cannot discriminate betwe...
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Article
Epidural spinal tumor and periaortitis as rare complications of Wegener’s granulomatosis
This case report describes findings in a 61-year-old woman who manifested scleritis, small pulmonary nodules, otitis media, periaortitis, and progressive epidural spinal tumor, associated with elevated serum m...
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Article
Role of imaging studies in the diagnosis and evaluation of giant cell arteritis in Japanese: report of eight cases
The objective of this study is to clarify the characteristics and imaging results of Japanese patients with giant cell arteritis (GCA). Eight patients with biopsy-proven GCA were enrolled. Their clinical data ...
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Article
Open AccessNonbacterial thrombotic endocarditis associated with cancer of unknown origin complicated with thrombus in the left auricular appendage: case report
A 63-year-old man was admitted to our hospital with a complaint of right lateroabdominal pain. He was diagnosed with metastatic colon cancer, and then developed multiple brain embolic infarctions 7 days after ...
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Article
Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation method
We developed a novel method to coat living non-genetically modified (GM) cells with functional recombinant proteins. First, we prepared GM yeast to secrete constructed proteins that have two domains: a functio...
