4 Result(s)
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Article
Open AccessMassively parallel whole genome amplification for single-cell sequencing using droplet microfluidics
Massively parallel single-cell genome sequencing is required to further understand genetic diversities in complex biological systems. Whole genome amplification (WGA) is the first step for single-cell sequenci...
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Article
Open AccessObtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes
Single-cell genomics is a straightforward approach to obtain genomes from uncultured microbes. However, sequence reads from a single-cell amplified genome (SAG) contain significant bias and chimeric sequences....
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Article
Open AccessHigh-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functio...
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Article
Open AccessStrain-level profiling of viable microbial community by selective single-cell genome sequencing
Culture-independent analysis with high-throughput sequencing has been widely used to characterize bacterial communities. However, signals derived from non-viable bacteria and non-cell DNA may inhibit its chara...
