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Article
Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development
Lipids are indispensable for energy storage, membrane structure and cell signalling. However, dynamic changes in various categories of endogenous lipids in mammalian early embryonic development have not been s...
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Chapter
Embryo Transfer
The success of assisted reproductive technology depends on maximizing the efficiency of each step. Embryo transfer (ET) is considered the final and critical step in the laborious process of in vitro fertilizat...
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Chapter
Osmolality of Culture Media
Gametes and embryos are highly sensitive to changes in culture media osmolality. Any fluctuation in culture media osmolality may adversely stress the gametes and embryos and obstruct their growth and developme...
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Chapter
Microbial Contamination of Culture Media
The IVF culture system provides a safe and stable environment for the survival and development of gametes and embryos in vitro. However, this stable, ideal environment is also hospitable to various microorgani...
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Chapter
Trophectoderm Biopsy
In 1990, Handyside et al. reported a successful pregnancy utilizing preimplantation genetic diagnosis (PGD) technology for embryo sex selection in IVF for the first time [1]. Nowadays, the technology has been ...
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Chapter
Temperature Control in IVF Culture System
Temperature influences the entire process of in vitro fertilization-embryo transfer. Today, most laboratories keep the embryo culture temperature at 37 °C, which is close to the human basal body temperature an...
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Chapter
Hydrogen Ion Concentration Index of Culture Media
The hydrogen ion concentration index, commonly referred to as “pH,” with “p” derived from the German word “Potenz” and “H” for hydrogen ion. The pH is the ratio of the total count of hydrogen ions to the total...
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Chapter
Light of Microscopes and Laboratory Environment
Oocytes development, ovulation, fertilization, and embryo development occur without exposure to light under physiological conditions in vivo. Although in vitro fertilization-embryo transfer technology is becom...
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Chapter
Morphological Evaluation of Cleavage-Stage Embryos and Blastocysts
Over the last two decades, methodologies for assessing embryo implantation potential have emerged, including time-lapse imaging systems, various “-omics” techniques (genomics, metabolomics, etc.), and artifici...
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Book
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Chapter
Quality Management of Intracytoplasmic Sperm Injection
Intracytoplasmic sperm injection (ICSI) is assisted reproduction laboratories’ most routine microscopic procedure. It includes a set of equipment and a technique and solves most infertility caused by male fact...
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Chapter
Introduction of Quality Control and Risk Management in IVF Laboratory
Assisted reproductive technology (ART) refers to medical interventions for infertile couples to have a baby. The assisted reproduction laboratory, also known as in vitro fertilization (IVF) laboratory, is an i...
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Chapter
Gas Supplies of Incubators, Air Quality, and Volatile Organic Compounds Management of the Laboratory Environment
A safe and stable environment for gamete and embryo culture is essential for assisted reproductive treatment, but this environment is influenced by multiple factors, one of which is gas. Regardless of ambient ...
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Chapter
Quality Management of Gamete and Embryo Cryopreservation
The cryopreservation of gametes and embryos is one of the routine practices within the IVF laboratory. The increasingly sophisticated gamete and embryo cryopreservation technology secure the safety and effecti...
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Article
Open AccessEffects of immediate versus delayed frozen embryo transfer in high responder patients undergoing freeze-all cycles
Frozen embryo transfer (FET) can greatly improve the pregnancy outcomes for high responder patients. However, it is not known whether the timing of FET is a risk factor on pregnancy outcomes in high responder ...
