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  1. No Access

    Article

    Practical considerations for quantitative light sheet fluorescence microscopy

    Fluorescence microscopy has evolved from a purely observational tool to a platform for quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic imaging modalities has spurre...

    Chad M. Hobson, Min Guo, Harshad D. Vishwasrao, Yicong Wu, Hari Shroff… in Nature Methods (2022)

  2. Article

    Open Access

    Incorporating the image formation process into deep learning improves network performance

    We present Richardson–Lucy network (RLN), a fast and lightweight deep learning method for three-dimensional fluorescence microscopy deconvolution. RLN combines the traditional Richardson–Lucy iteration with a ...

    Yue Li, Yijun Su, Min Guo, **aofei Han, Jiamin Liu, Harshad D. Vishwasrao… in Nature Methods (2022)

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  3. No Access

    Article

    Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes

    We demonstrate residual channel attention networks (RCAN) for the restoration and enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data. First we modify RCAN to handle image volu...

    Jiji Chen, Hideki Sasaki, Hoyin Lai, Yijun Su, Jiamin Liu, Yicong Wu… in Nature Methods (2021)

  4. Article

    Author Correction: Faster, sharper, and deeper: structured illumination microscopy for biological imaging

    In the version of this Perspective originally published, Fig. 4g included an incorrect inset adapted from a different figure than the main image in the panel. This error has been corrected in the PDF and HTML ...

    Yicong Wu, Hari Shroff in Nature Methods (2019)

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  5. No Access

    Article

    Faster, sharper, and deeper: structured illumination microscopy for biological imaging

    Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Sin...

    Yicong Wu, Hari Shroff in Nature Methods (2018)

  6. No Access

    Article

    Single-shot super-resolution total internal reflection fluorescence microscopy

    We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatia...

    Min Guo, Panagiotis Chandris, John Paul Giannini, Adam J. Trexler… in Nature Methods (2018)

  7. No Access

    Article

    Adaptive optics improves multiphoton super-resolution imaging

    Adaptive optics and two-photon instant structured illumination microscopy are combined to provide improved super-resolution imaging within optically aberrating biological samples.

    Wei Zheng, Yicong Wu, Peter Winter, Robert Fischer, Damian Dalle Nogare… in Nature Methods (2017)

  8. No Access

    Article

    Dual-view plane illumination microscopy for rapid and spatially isotropic imaging

    Kumar et al. describe how to build a diSPIM from commercially available parts and use it to live-image cultured cells or worm embryogenesis. The inverted setup enables samples to be mounted directly on microscope...

    Abhishek Kumar, Yicong Wu, Ryan Christensen, Panagiotis Chandris… in Nature Protocols (2014)