8 Result(s)
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Article
Open AccessProtrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation
Endosome maturation depends on membrane contact sites (MCSs) formed between endoplasmic reticulum (ER) and endolysosomes (LyLEs). The mechanism underlying lipid supply for this process and its pathophysiologic...
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Article
Open AccessMyristoyl group-aided protein import into the mitochondrial intermembrane space
The MICOS complex mediates formation of the crista junctions in mitochondria. Here we analyzed the mitochondrial import pathways for the six yeast MICOS subunits as a step toward understanding of the assembly ...
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Article
Open AccessCdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo
MPIase is a glycolipid that is involved in membrane protein integration. Despite evaluation of its functions in vitro, the lack of information on MPIase biosynthesis hampered verification of its involvement in vi...
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Open AccessVisualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system
Functional integrity of eukaryotic organelles relies on direct physical contacts between distinct organelles. However, the entity of organelle-tethering factors is not well understood due to lack of means to a...
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Article
Open AccessQuality control of nonstop membrane proteins at the ER membrane and in the cytosol
Since messenger RNAs without a stop codon (nonstop mRNAs) for organelle-targeted proteins and their translation products (nonstop proteins) generate clogged translocon channels as well as stalled ribosomes, ce...
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Article
Open AccessA phospholipid transfer function of ER-mitochondria encounter structure revealed in vitro
As phospholipids are synthesized mainly in the endoplasmic reticulum (ER) and mitochondrial inner membranes, how cells properly distribute specific phospholipids to diverse cellular membranes is a crucial prob...
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Article
Open AccessStructural and mechanistic insights into phospholipid transfer by Ups1–Mdm35 in mitochondria
Eukaryotic cells are compartmentalized into membrane-bounded organelles whose functions rely on lipid trafficking to achieve membrane-specific compositions of lipids. Here we focused on the Ups1–Mdm35 system, ...
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Article
Ubiquitin is phosphorylated by PINK1 to activate parkin
Ubiquitin, known for its role in post-translational modification of other proteins, undergoes post-translational modification itself; after a decrease in mitochondrial membrane potential, the kinase enzyme PIN...
