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Article
FTSJ3 is an RNA 2′-O-methyltransferase recruited by HIV to avoid innate immune sensing
In mammals, 2′-O-methylation of RNA is a molecular signature by which the cellular innate immune system distinguishes endogenous from exogenous messenger RNA1–3. However, the molecular functions of RNA 2′-O-methy...
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Article
Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels
Exportin-5 is involved in microRNA precursor nuclear export. Dicer mRNA is now found to compete with pre-miRNAs for exportin-5 binding, suggesting that cytoplasmic levels of Dicer and its pre-miRNA substrate e...
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Article
Open AccessSuppression of HIV-1 replication by microRNA effectors
The rate of HIV-1 gene expression is a key step that determines the kinetics of virus spread and AIDS progression. Viral entry and gene expression were described to be the key determinants for cell permissiven...
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Article
RNAi Therapy for HIV Infection
Inside eukaryotic cells, small RNA duplexes, called small interfering RNAs (siRNAs), activate a conserved RNA interference (RNAi) pathway which leads to specific degradation of complementary target mRNAs throu...
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Article
Open AccessHIV-1 Tat interaction with Dicer: requirement for RNA
Dicer is an RNase III which processes two classes of cellular small RNAs: the microRNAs (miRNA) and short interfering RNAs (siRNA). Previously, we observed that over-expressed HIV-1 Tat protein can suppress th...
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Protocol
MicroRNAs in Human Immunodeficiency Virus-1 Infection
Initially reported for Caenorhabditis elegans, microRNA (miRNA) has been shown to regulate gene expression in plants, flies, and mammals (1,2). Recently, findings of miRNA have been extended to viruses (3,4). Her...
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Article
Open AccessChanges in microRNA expression profiles in HIV-1-transfected human cells
MicroRNAs (miRNAs) are small RNAs of 18–25 nucleotides (nt) in length that play important roles in regulating a variety of biological processes. Recent studies suggest that cellular miRNAs may serve to control...
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Article
siRNA, miRNA and HIV: promises and challenges
Small interfering RNA (siRNA) and microRNA (miRNA) are small RNAs of 18-25 nucleotides (nt) in length that play important roles in regulating gene expression. They are incorporated into an RNA-induced silencin...
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Article
Open AccessHIV-1 encoded candidate micro-RNAs and their cellular targets
MicroRNAs (miRNAs) are small RNAs of 21–25 nucleotides that specifically regulate cellular gene expression at the post-transcriptional level. miRNAs are derived from the maturation by cellular RNases III of im...
