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Article
Sphingomonas beigongshangi sp. nov., Isolated from Pit Mud of Baijiu
Two novel aerobic, gram-negative, rod-shaped bacterial strains designated strains REN5T and REN5-1, were isolated from pit mud of Baijiu in Sichuan province, China. Based on the 16S rRNA gene sequence similarity,...
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Article
Identification of Phosphorylated Proteins in Response to Salt Stress in Wheat Embryo and Endosperm during Seed Germination
Seed germination is a new beginning for the crop life cycle, which is closely related to seed sprouting and subsequent plant growth and development, and ultimately affects grain yield and quality. Salt stress ...
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Article
Open AccessSynthesis and Accumulation of Glutenin Subunits during Grain Development in Bread Wheat as Revealed by Reversed-phase Ultra-performance Liquid Chromatography (RP-UPLC)
Wheat glutenins containing high and low molecular weight glutenin subunits (HMW-GS and LMW-GS) are the major determinants of wheat gluten quality. In this study, the recently developed reversed-phase ultra-per...
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Article
Transcription factor C/EBP-β induces tumor-suppressor phosphatase PHLPP2 through repression of the miR-17–92 cluster in differentiating AML cells
PHLPP2, a member of the PH-domain leucine-rich repeat protein phosphatase (PHLPP) family, which targets oncogenic kinases, has been actively investigated as a tumor suppressor in solid tumors. Little is known,...
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Article
Open AccessComparative Proteomic Analysis of Wheat Develo** Grains between Chinese Spring and 1Sl/1B Substitution Line
A comparative proteomic analysis of grain proteins during five grain developmental stages of wheat cultivar Chinese Spring (CS) and its 1Sl/1B substitution line CS-1Sl(1B) was carried out in the current study. A ...
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Article
Exogenous salicylic acid improves salinity tolerance of Nitraria tangutorum
In the present study, the physiological responses of Nitraria tangutorum Bobr. seedlings to NaCl stress and the regulatory function of exogenous application of salicylic acid (SA) were investigated. NaCl in low c...
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Article
Cloning, expression, and characterization of phyA gene from Ipomoea batatas
Phytochrome A (phyA), as a photoreceptor, has a profound influence on plant growth and development and participates in many important physiological processes, such as seed germination, seeding deetiolation, an...
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Article
Open AccessApplications of Capillary Electrophoresis for Rapidly Separating and Characterizing Water-soluble Proteins of Wheat Grains
Separation and analysis of water-soluble proteins (WSP) are important in understanding wheat grain proteome fundamentals. However, due to their high degree of heterogeneity and complexity in the compositions, ...
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Article
Identification of conserved MicroRNAs and their targets in Phalaenopsis orchid
The microRNAs (miRNAs) are a new type of tiny, noncoding, single-stranded endogenous RNA molecules performing their function of regulating gene expression by targeting mRNAs for degradation or restraining prot...
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Article
Open AccessIsolation and expression of a new high molecular weight glutenin subunit gene at the Glu-D-1-2 locus from Aegilops tauschii
Two new y-type HMW-GSs in Ae. tauschii, 1Dy12.1*t and 1Dy12.2t with the mobility order of 1Dy12.2t > 1Dy12.1*t > 1Dy12.1t>1Dy12, were identified by both SDS-PAGE and MALDI-TOF-MS. Molecular cloning and sequencing...
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Article
Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.)
Three novel low molecular weight (LMW) glutenin subunits from cultivated einkorn (Triticum monococcum L., AmAm, 2n = 2x = 14) were characterized by SDS-PAGE and molecular weights determined by MALDI-TOF-MS. Their...
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Article
Identification and molecular characterization of a novel y-type Glu-D t 1 glutenin gene of Aegilops tauschii
A novel y-type high-molecular-weight glutenin subunit possessing a slightly faster mobility than that of subunit 1Dy12 in SDS-PAGE, designated 1Dy12.1t in Aegilops tauschi, was identified by one- and two-dimensio...
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Article
HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelt wheat (Triticum spelta L.) progenitors
The allelic compositions of high- and low-molecular-weight subunits of glutenins (HMW-GS and LMW-GS) among European spelt (Triticum spelta L.) and related hexaploid and tetraploid Triticum species were investiga...
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Article
Biochemical characterization and bacterial expression of an odorant-binding protein from Locusta migratoria
Analysis of soluble proteins from different body parts of Locusta migratoria revealed a fast-migrating component in native electrophoresis, unique to antennae of both sexes. N-terminal sequence analysis and clon...
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Article
Increased expression of hepatic DNA methyltransferase in smokers
The DNA methyltransferase enzyme (DNA MTase) catalyzes DNA methylation at cytosines in CpG dinucleotides. 5-Methylcytosine modification of DNA is important in gene regulation, DNA replication, chromatin organi...
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Article
Studies on Genetic Control of Low-Molecular-Weight Glutenin Subunits in Wheat Endosperm by A-PAGE Analysis
Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated usi...