55 Result(s)
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Article
Determination of neodymium, holmium and erbium in mixed rare earths by norfloxacin
Norfloxacin (NFX) is proposed as reagent for the derivative spectrophotometric determination of neodymium, holmium and erbium in mixed rare earths. The absorption spectra of 4f electron transitions of the sys...
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Expression and purification of a mutant of human interleukin-2 in Pichia pastoris
Interleukin (IL)-2 is a pharmacologically important cytokine secreted by T-lymphocytes. Recombinant IL-2 (rIL-2) has been modified and produced in many systems. Mass production of rIL-2 is the prerequisite for...
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Molecular Cloning, Sequencing, and Expression of a l-Glutamine d-Fructose 6-Phosphate Amidotransferase Gene from Volvariella volvacea
Using 3′-RACE and 5′-RACE, we have cloned and sequenced the genomic gene and complete cDNA encoding l-glutamine d-fructose 6-phosphate amidotransferase (GFAT) from the edible straw mushroom, Volvariella volvacea....
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Article
Enhancing the Enzymatic Hydrolysis of Corn Stover by an Integrated Wet-milling and Alkali Pretreatment
An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition,...
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Erratum to: Enhancing the Enzymatic Hydrolysis of Corn Stover by an Integrated Wet-milling and Alkali Pretreatment
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Article
Isolation, Purification, and Properties of a Novel Small Heat Shock Protein from the Hyperthermophile Sulfolobus solfataricus
The isolation, purification, and properties of a putative small heat shock protein (sHsp), named SsHSP14.1, from the hyperthermophilic archaeon Sulfolobus solfataricus have been investigated. The sHsp was success...
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Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis
The chemical composition of herbal medicines is very complex, and their therapeutic effects are determined by multi-components with sophisticated synergistic and/or suppressive actions. Therefore, quality cont...
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Selenium Enrichment on Cordyceps militaris Link and Analysis on Its Main Active Components
To investigate the effects of selenium on the main active components of Cordyceps militaris fruit bodies, selenium-enriched cultivation of C. militaris and the main active components of the fruit bodies were stud...
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Article
Surface Plasmon Resonant THz Wave Transmission on Carbon Nanotube Film
The properties of the terahertz resonant surface plasmons wave on the carbon nanotube film and dielectric interface have been investigated. As a first step towards engineering terahertz SPPs-like surface modes...
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Article
Immobilization of Pseudomonas fluorescens Lipase onto Magnetic Nanoparticles for Resolution of 2-Octanol
The lipase from Pseudomonas fluorescens (Lipase AK, AKL) was immobilized onto the magnetic Fe3O4 nanoparticles via hydrophobic interaction. Enzyme loading and immobilization yield were determined as 21.4 ± 0.5 mg...
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Article
One-Step Preparation and Fluorescence Enhancement Effect in SiO2/Eu2+ Doped with Ag Nanowires
Eu2+ single-doped SiO2 (SiO2/Eu2+) and Eu2+, Ag nanowires co-doped SiO2 (SiO2/Eu2+–Ag) luminescent nanomaterials were prepared by an efficient one-step sol–gel method. Their microstructure and optical properties ...
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In vitro metabolic study of Rhizoma coptidis extract using liver microsomes immobilized on magnetic nanoparticles
Although metabolic study of individual active compounds isolated from herbal plants has been intensive, it cannot truly reflect the fate of herbs because the herbal extracts in use have many constituents. To a...
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Article
The Relationship of Freeze Tolerance with Intracellular Compounds in Baker’s Yeasts
Freeze-tolerant baker’s yeasts are required for the processing of frozen doughs. The present study was carried out to investigate the cell survival rate after frozen storage and the change of fermentability in...
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Article
Optimization of Culture Conditions for Enhanced Lysine Production Using Engineered Escherichia coli
In this study, culture conditions, including dissolved oxygen (DO) content, presence of osmoprotectants, residual glucose concentration, and ammonium sulfate-feeding strategies, were investigated for decreasin...
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Article
Production, Purification, and Characterization of a Cellulase-Free Thermostable Endo-xylanase from Thermoanaerobacterium thermosaccharolyticum DSM 571
This is the first report describing the cloning, expression, and characterization of a putative thermostable, cellulase-free xylanase (XYN) from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyti...
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Article
Functional inactivation of lymphocytes by methylene blue with visible light
Transfusion of allogeneic white blood cells (WBCs) may cause adverse reactions in immunocompromised recipients, including transfusion-associated graft-versus-host disease (TA-GVHD), which is often fatal and incur...
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Article
Analysis of Asymmetric Dipoles Interacting in Heterogeneous Metal Nanorod Dimers
The localized electric field enhancement of Au-Ag nanorods dimer is theoretically investigated based on finite element method (FEM). An analytical mutual-interference model between asymmetric dipole-dimer has ...
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Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism
Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was...
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Article
Power consumption and gas—liquid dispersion in turbulently agitated vessels with vertical dual-array tubular coil baffles
In this work vertical dual-array tubular coil baffles arranged in groups of four, six or eight were investigated and the results compared with those from four planar baffles. The baffle coefficients for a sing...
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Article
Open AccessDirected assembly of cobalt(II) 1-H-indazole-3-carboxylic acid coordination networks by bipyridine and its derivatives: structural versatility, electrochemical properties, and antifungal activity
This paper describes the hydrothermal synthesis, full characterization, and architectural diversity of three intriguingly bioactive cobalt–organic frameworks, namely, 3D [Co(HL ...
