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  1. Article

    Open Access

    High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

    pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the appl...

    **ngYa Zhao, JianYong Xu, Ming Tan, Jie Zhen, WenJu Shu… in Microbial Cell Factories (2020)

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  2. No Access

    Article

    High-level extracellular production of an alkaline pectate lyase in E. coli BL21 (DE3) and its application in bioscouring of cotton fabric

    A high heterologous expression of an alkaline pectate lyase (APL) pelNK93I in E. coli was obtained through optimizing the lactose feeding and fed-batch fermentation. The highest soluble APL activity produced by E...

    Jie Zhen, Ming Tan, ** Fu, Wenju Shu, **ngya Zhao, Shibin Yang… in 3 Biotech (2020)

  3. No Access

    Article

    Ethanol effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3)

    A novel method involving ethanol-induced increase in the heterologous recombinant protein expression in E. coli cells was commonly used in recent studies. However, the detailed mechanism of this method is still t...

    Hongchen Zheng, Zhenxiao Yu, Wenju Shu… in Applied Microbiology and Biotechnology (2019)