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Article
Structural insights into vesicular monoamine storage and drug interactions
Biogenic monoamines—vital transmitters orchestrating neurological, endocrinal and immunological functions1–5—are stored in secretory vesicles by vesicular monoamine transporters (VMATs) for controlled quantal rel...
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Article
Open AccessThe characteristic patterns of individual brain susceptibility networks underlie Alzheimer’s disease and white matter hyperintensity-related cognitive impairment
Excessive iron accumulation in the brain cortex increases the risk of cognitive deterioration. However, interregional relationships (defined as susceptibility connectivity) of local brain iron have not been ex...
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Article
Stabilization and structure determination of integral membrane proteins by termini restraining
Integral membrane proteins isolated from cellular environment often lose activity and native conformation required for functional analyses and structural studies. Even in their native state, they lack sufficie...
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Article
Machine learning based on the multimodal connectome can predict the preclinical stage of Alzheimer’s disease: a preliminary study
Subjective cognitive decline (SCD) may be a preclinical stage of Alzheimer’s disease (AD). Neuroimaging studies suggest that abnormal brain connectivity plays an important role in the pathophysiology of SCD. H...
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Article
Open AccessNanoparticles and photochemistry for native-like transmembrane protein footprinting
Mass spectrometry-based footprinting can probe higher order structure of soluble proteins in their native states and serve as a complement to high-resolution approaches. Traditional footprinting approaches, ho...
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Article
Fabrication of Cu/graphite film/Cu sandwich composites with ultrahigh thermal conductivity for thermal management applications
Effective thermal management of electronic integrated devices with high powder density has become a serious issue, which requires materials with high thermal conductivity (TC). In order to solve the problem of...
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Article
Redox-controlled generation of the giant porphyry Cu–Au deposit at Pulang, southwest China
Some porphyry Cu–Au deposits with relatively reduced ore assemblages, characterized by high hydrothermal pyrrhotite contents and a lack of primary hematite and magnetite, are generally considered to be associa...
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Article
Warfarin traps human vitamin K epoxide reductase in an intermediate state during electron transfer
Mass spectrometry and biochemical analyses reveal that the major form of VKOR found in cells features a disulfide bond between Cys51 and Cys132, and this intermediate is the target of the anticoagulant drug wa...
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Article
Structure of the SecY channel during initiation of protein translocation
Newly synthesized proteins are targeted to the SecY protein-conducting channel for translocation across the membrane; here, cryo-electron microscopy structures of inactive and active ribosome–channel complexes...
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Article
Structures of an intramembrane vitamin K epoxide reductase homolog reveal control mechanisms for electron transfer
The intramembrane vitamin K epoxide reductase (VKOR) supports blood coagulation in humans and is the target of the anticoagulant warfarin. VKOR and its homologues generate disulphide bonds in organisms ranging...
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Article
Structure of a bacterial homologue of vitamin K epoxide reductase
Vitamin K epoxide reductase (VKOR) generates vitamin K hydroquinone to sustain γ-carboxylation of many blood coagulation factors. Here, we report the 3.6 Å crystal structure of a bacterial homologue of VKOR from
